Germplasm Resources Characteristics And Production Performance Analysis Of Dongda Sika Deer

Due to the small population size and closed management,there may be genetic drift effects and inbreeding phenomena within the artificially captive sika deer population,which reduces the genetic diversity of the population,leading to a decrease in adaptability to environmental conditions,disease resistance,and population quality,causing direct economic losses to the deer breeding industry.Therefore,this study takes the Dongda Sika deer population as the research object,aiming to use microsatellite markers to analyze the genetic diversity of Dongda Sika deer,in order to more effectively breed and protect variety resources,and provide scientific basis for the breeding,management,and breeding improvement of Dongda Sika deer population.At the same time,screening microsatellite marker loci related to the antler weight trait of Dongda Sika deer provides molecular basis for genetic breeding and trait improvement of Dongda Sika deer.In this experiment,11 microsatellite loci were selected and 8 of them were selected with good polymorphism.They were used to detect the genetic diversity of 60 Dongda sika deer.After PCR amplification,capillary electrophoresis was used for genotyping.SPSS statistical software was used to analyze the correlation between different genotypes of 8 microsatellite loci and the antler weight traits of Dongda sika deer.The results show that:In terms of microsatellite genetic diversity,parameters such as effective allele number,observed heterozygosity,polymorphism information content,and Shannon information index were analyzed.A total of 77 alleles were detected in the Dongda sika deer population,with an average of 9.625 per locus,of which BM1225 was the largest,with 14 alleles;The minimum number of loci T108 is 5.The effective allele number of the eight microsatellite loci in Dongda sika deer ranged from 1.6537 to 7.362,with an average of 3.9481,with the highest number of loci BM1225 and the lowest number of loci T108.The observed heterozygosity ranged from 0.1333 to 0.8167,with an average of 0.5744.T123 and T156 are the highest and T108 is the lowest.The expected heterozygosity is between 0.3986 and 0.8714,with an average of 0.6802.BM1225 is the highest and T108 is the lowest.The polymorphism information content ranged from 0.3349 to0.8509,with an average value of 0.6446,with the highest at locus BM1225 and the lowest at locus T108.The Shannon information index ranges from 0.6809 to 2.2408,with an average of 1.5428.Among them,BM1225 is the highest and T108 is the lowest.The results showed that only BM1706,T123,and T530 loci met the Hardy Weinberg equilibrium(P>0.05).The EwensWatterson neutral test was conducted on 8 microsatellite loci in the Dongda sika deer population.The results showed that the observed F values of the 8 sites were within the 95% confidence interval and belonged to neutral sites.The F-statistics of the Dongda sika deer population show that Fis is negative at each point,with an overall average of-1.Fit showed a negative value at site T156 and a positive value at other sites,with an overall average of 0.1981.Fst is positive at each point,with an overall average of 0.5991.The gene flow at each point ranges from 0.0507 to0.2597,with an average of 0.1808.This indicates that the Dongda sika deer population has rich genetic diversity.The correlation analysis between 8 microsatellite loci and antler weight traits showed that the EE genotype of T123 microsatellite loci was significantly correlated with individual antler yield(P<0.05).It can be used as a reference site for breeding improvement of Dongda sika deer.