| As a traditional medicinal plant,Paeonia Radix Rubra(PRR)root has a long history of cultivation in China,and is an important part of many compound preparations.In recent years,with the continuous development of medicinal value of PRR root,the demand for PRR root is increasing,and the yield of wild PRR root is difficult to meet the market demand,so a large number of artificial cultivation of high quality PRR root is needed.Therefore,taking PRR root as the research object,through studying the changes of soil physical and chemical properties and secondary metabolites of PRR root during cultivation under the condition of earthworm manure fertilizer combination,the most appropriate fertilization method of PRR root was found,which provided the experimental basis for standardized artificial cultivation of PRR root.Under the combined application mode of earthworm manure fertilizer,experimental treatment: No fertilizer treatment(CK),low quantity of vermicompost treatment(V1)and high quantity of vermicompost treatment(V2),three fertilizer treatment(P1,P2,P3),and fertilizer and vermicompost combined treatment(V1P1,V1P2,V1P3,V2P1,V2P2,V2P3),The content of soil nutrients and the content of secondary metabolites in root of PRR root were studied in 0-20 cm tilth layer during the growth stage,flowering stage,dormant stage and maturity stage,and the main results were as follows:(1)Application of vermicompost could significantly increase the content of soil organic matter.Compared with CK,the content of soil organic matter in V2 treatment and V1 treatment increased by20.92% and 16.98%,respectively,reaching 60.28 g/kg and 58.17 g/kg.The content of soil organic matter in the vermicompost combined with chemical fertilizer V2P2 treatment is 59.55 g/kg,which is19.46% higher than that in CK.At the same time,the contents of total nitrogen,alkali-hydrolyzed nitrogen,available phosphorus,available potassium and p H of soil were significantly increased by application of vermicompost(p<0.05).The contents of total nitrogen(1.99 g/kg),alkali-hydrolyzed nitrogen(293.56 mg/kg)and available phosphorus(71.05 mg/kg)in soil treated by V2P2 were the highest,which were 23.89%,23.83% and 108.99% higher than those of CK,respectively.The highest available potassium content in V1P2 treatment was 260.70 mg/kg.The p H of soil treated by V1(6.03)was the highest,which increased by 0.35 compared with CK.(2)Earthworm manure combined with fertilizer V2P2(45.15%)had the greatest effect on root drying rate of PRR root,which was 10.09% higher than CK.The second was V1P2(44.60%),which increased by 8.75% compared with CK.The drying rate of other treatment groups increased by5.00-8.56%.(3)Vermicompost significantly increased the content of secondary metabolites in root system of PRR root,but significantly decreased the content of benzoic acid(p<0.05).The content of secondary metabolites such as oxypaeoniflorin,catechin,Paeoniflorin,Paeoniflorin and Paeoniflorin in V2P2 treatment group increased by 125.75%,211.44%,99.79% and 38.24% compared with CK,respectively.The contents of 1,2,3,4,6-o-gallic glucose and benzoyl paeoniflorin in V1P2 treatment group were increased by 169.07% and 63.00% compared with CK,which were significantly higher than other treatments.Therefore,the best fertilization method for Paeony is high amount of vermicompost combined with medium amount of fertilizer.(4)A canonical correlation analysis model was established to investigate the relationship between soil nutrients and root secondary metabolites of PRR root after fertilization.The results showed that soil organic matter and alkali-hydrolyzed nitrogen were positively correlated with paeoniflorin,gallic acid,oxypaeoniflorin and benzoyl paeoniflorin. |
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