Effect Of Hydrogen Peroxide Combined With Bacterial Manure On Replanted Malus Hupehensis Rehd.seedlings

Pot experiment was conducted to explore the effects of applying bacterial manure to apple replanted soil after treatment with strong oxidizing agent on the environmental changes of replanted soil and replanting of sweet tea seedlings in order to provide new technology for alleviating Apple replant disease.A total of nine treatments were set in the study: apple replanted soil(CK1),methyl bromide fumigation(CK2),hydrogen peroxide(3% concentration)treatment(CK3),hydrogen peroxide plus liquid bacterial manure of Bacillus Subtilis XC1(T1),hydrogen peroxide plus liquid bacterial manure of Trichoderma Echinosporum 6S-2(T2),hydrogen peroxide plus liquid bacterial manure of Bacillus Subtilis XNRB-3(T3),The hydrogen peroxide treatment is followed by the application of a sterilized Bacillus Subtilis XC1 liquid bacterial fertilizer(T4),the hydrogen peroxide treatment is followed by the application of a sterilized Trichoderma Echinosporum 6S-2 liquid bacterial fertilizer(T5),and the hydrogen peroxide treatment is followed by the sterilization of a Bacillus Subtilis XNRB-3 liquid bacterial fertilizer(T6).The main test results are as follows:1.Application of bacterial manure after hydrogen peroxide treatment was more conducive to improving the biomass of replanted Malus hupehensis Rehd.seedlings.Compared with the replanted control(CK1),the application of bacterial manure after hydrogen peroxide treatment significantly increased the biomass of replanted plants.In July and September 2022,compared with replanted control(CK1),the plant height,ground diameter and fresh weight of treatment T1 were increased significantly by 58.52%,6.15%,168.12% and 23.04%,19.89% and 118.52%,respectively.Plant height,ground diameter and fresh weight increased by 62.01%,24.08%,157.05% and 21.12%,34.07% and 110.36% respectively under T2 treatment.The plant height,ground diameter and fresh weight of T3 treatment were significantly increased by 60.88%,41.30%,169.00% and 22.84%,25.70% and 119.58%,respectively.2.Application of bacterial manure after hydrogen peroxide treatment was more conducive to improving the root architecture-related index,enhancing the activity of root protective enzymes,enhancing root activity,and reducing MDA content.Under different treatment conditions,the overall gradient of root architecture-related index,root protective enzyme activity and root activity was > hydrogen peroxide+bacterial manure treatment(T1,T2,T3)> hydrogen peroxide+inactivated bacterial manure treatment(T4,T5,T6)> hydrogen peroxide treatment(CK3)> replanted control(CK1).MDA content was showed as bromomethane treatment and hydrogen peroxide and their related treatments(CK2,CK3,T1,T2,T3,T4,T5,T6)< replanted control(CK1)3.Hydrogen peroxide treatment reduced soil enzyme activity and soil phenolic acid content,and subsequent application of bacterial manure could further improve soil enzyme activity and reduce soil phenolic acid content.The activities of sucrase,neutral phosphatase,catalase and urease showed an overall trend of treatment with bacterial manure after hydrogen peroxide treatment(T1,T2 and T3)> treatment with inactivated bacterial manure after hydrogen peroxide treatment(T4,T5 and T6)> replanted control(CK1)> bromomethane treatment(CK2)and hydrogen peroxide treatment(CK3).Compared with the replanted control(CK1),the treatment with hydrogen peroxide plus bacterial manure(T1,T2,and T3)significantly reduced the phloridzin by 72.16%,61.76%,and 75.10%,respectively.The content of phloretin was decreased by 75.96%,68.74% and 77.91% respectively.Benzoic acid decreased 48.77%,41.00% and 57.72% respectively;P-hydroxybenzoic acid decreased by 48.92%,43.41% and 53.81% respectively;Cinnamic acid was significantly reduced by 52.29%,39.57% and 54.63%,respectively.4.Hydrogen peroxide can reduce the number of soil microorganisms and harmful fungi,and the application of bacterial manure further reduces the number of soil fungi and increases the number of soil bacteria.The results of real-time fluorescent quantitative PCR and highthroughput sequencing indicated that hydrogen peroxide plus bacterial manure treatment reduced the relative abundance of Fusarium and the gene copy numbers of four key pathogenic Fusarium species(Fusarium oxysporum,Fusarium moniliforme,Fusarium oxysporum,Fusarium oxysporum),significantly increased the relative abundance of Trichoderma and optimized the microbial community structure of the soil.Hydrogen peroxide plus XNRB-3 was the best.