Study On Adaptability And Phylogenetic Relationship Of Ampelopsis Grossedentata To Karst Soils Under Artificial Conditions

At present,the control of rocky desertification in karst areas of our country has achieved remarkable results,but the main contradiction of rocky desertification areas,that is,the solution of the contradiction between man and land,is the fundamental problem of control.How to integrate ecological restoration and economic development in repairing karst areas and controlling rocky desertification has become a mainstream issue in the present and future.Based on the principle of treating the symptom and treating the root cause,this paper sought feasibility in the experience of many liana plants to control rocky desertification,and finally selected the liana species of the Ampelopsis grossedentata as the research object.Firstly,we investigated the changes of plant growth and development,functional components and physiological metabolism of Ampelopsis grossedentata under different artificial calcium ion environment and karst soil conditions with different calcium content.The contents of eight characteristic products,including total flavonoids,proline,malondialdehyde,chlorophyll and soluble sugar,were determined,which provided a solid foundation for the restoration of the ecological environment in karst rocky desertification areas,the promotion of emerging dominant species resources according to local conditions,the sustainable development of local economy,and the complete elimination of the threat of rocky desertification.Through the chloroplast genome analysis,we compared the genetic distance and evolutionary differences among related species,which is of great significance for the maintenance of biodiversity,the promotion of geo-species,social market issues,and future breeding improvement in the process of rocky desertification control.The main results are as follows:(1)A.grossedentata is not a calcium-phobic plant.Wild plants could grow normally under the conditions of 4 and 25 mmol/L calcium,which were higher than the native soil calcium content.It is well adapted to the transition of different calcium ion environment from 4 to 100 mmol/L,and its tolerance to calcium ion is significantly increased..(2)The growth condition of A.grossedentata plants in the artificial environment of dolomite attached soil(calcium ion content reached35g/Kg,higher than that in non-karst soil)was not inferior to that in the native soil,and the content of functional flavonoid compounds dihydromyricetin and myricetin was equal to or even higher than that in the native soil.However,the growth condition was poor in the limestone attached soil(calcium ion content was up to 76g/Kg,which was higher than that in the non-karst soil).The plant was short and the leaves were sparse,but it could still survive.(3)Except for proline,malondialdehyde and chlorophyll,there were no significant differences in the contents of dihydromyricetin,myricetin,myricetin,total flavonoids and soluble sugar among the three genetic backgrounds(P > 0.05).However,the contents of flavonoids such as dihydromyricetin and myricetin in karst soil were higher than those in native soil under the medium calcium concentration in dolomite attached soil.However,the contents of soluble sugar and malondialdehyde,which directly reflected the stress degree of plants,were higher than those of native soil-cultivated plants.These results indicated that A.grossedentata could tolerate high Ca2? environment well and could adapt to or even take advantage of the unconventional Ca2? environment without death.(4)The plants of the A.megalophylla、A.cantoniensis are closely related to A.grossedentata.The whole chloroplast genome of A.grossedentata contained 134 genes and 89 protein-coding genes.In contrast,the whole chloroplast genomes of A.megalophylla and A.cantoniensis both have 131 genes and 86 protein-coding genes,and their GC content,repeat number,simple repeat sequences and other characteristics are also very similar.A.megalophylla is not only closely related,but also has almost the same main functional component,which is dihydromyricetin.Therefore,A.megalophylla can be considered as another ecological management model plant after A.grossedentata,and can also be considered as the plant extraction material of dihydromyricetin.In addition,other related species of Ampelopsis and even other related species of Vitaceae can also be included in the potential dominant species of ecological management in karst environment.