Study On The Rule Of Residual Elimination Of Tiamulin Fumarate Premix In Chickens

Tiamulin is a truncated lateral octin antibiotic,mainly used in the prevention and treatment of respiratory diseases and livestock and promote growth,is one of the widely used veterinary antibiotics.The drug is rapidly absorbed in animals and has strong antibacterial activity.It has good antibacterial activity against mycoplasma,bacteria and spirochetes,such as Mycoplasma subipneumoniae,Mycoplasma synovium,Mycoplasma septicum,streptococcus,etc.Low doses of teomin can promote animal growth and improve feed utilization,so it is widely used in livestock and poultry breeding.Although temicin is an animal special antibiotic,if the rest period is not strictly implemented in the breeding process,it may cause drug residues in livestock and poultry products to exceed the standard,and can enter the human body through the food chain,causing potential harm to classified health.The preparations approved for clinical use in China mainly include soluble powder and fumarate premixture.Among them,teilin is approved for pigs and chickens in foreign countries,while it is only approved for pigs in China,and there is no relevant regulation on the rest period in chickens,and there is still no research on the residual law of teilin chickens in China.Due to the broad application prospect of premixture in chicken production,it is very necessary to study the residual elimination law of the drug in veterinary medicine and develop the scientific and reasonable drug rest period.Therefore,the following several studies were conducted in this paper.1.A liquid chromatography-tandem mass spectrometry method for the determination of tiamulin residue markers(8-α-hydroxy miaolin)in chicken tissues was establishedThe residue of Typomycin in the tissue was extracted with a mixed solution of acetone-hydrochloric acid,and its alkali was hydrolyzed to 8-α-hydroxymiaolin,extracted by ethyl acetate,determined by liquid chromatography-tandem mass spectrometry,and the matrix was corrected by adding a standard solution,and the standard amount was exceeded.The results showed that the linear relationship between 8-α-hydroxy miaolin in the range of 25 μg/kg to 5000 μg/kg,and the correlation coefficient was greater than 0.99;the detection limit of muscle and sebum+ lipid tissue in this method was 25 g/kg,the quantitative limit was 50 g/kg,the quantitative limit of liver tissue was 50 g/kg,and the quantitative limit was 100 g/kg;the detection limit of 8-α-hydroxy miaolin was 50 μg/kg~200 μg/kg in muscle and skin + lipid,and 100 μg/kg~2000 μg/ kg in chicken liver.In the range of kg addition concentrations,the average recovery rate ranged from 82.1% to 105.7%,and the relative standard deviation ranged from 0.6% to 9.5%.This method is fast and simple,highly exclusive and sensitive,in line with the relevant provisions of veterinary drug residue detection,and can meet the requirements of the residue detection of Taimulin marker(8-α-hydroxy miaolin)in chicken tissue.2.Using the established 8-α-hydroxy miaolin residue detection method,the residue elimination test of tiamulin fumarate premix in chickens was carried out,and the drug suspension period was formulatedAccording to the "Guidelines for the Elimination of Veterinary Drug Residues",110 healthy white-feathered broilers weighing about 1 kg were selected,and 500 g of chickens(measured by Tiamulin fumarate)were fed according to each 1T feed,for 5consecutive days,4h,1d,2d,3d,5d and 7th days after stopping the drug were randomly bloodied and slaughtered 15 chickens.The content of the 8-α-hydroxy myolin determination method of the residual marker of Tiamulin established in this study was used to determine the content.The results showed that the residual amount of 8-α-hydroxy miaolin in liver tissue was significantly higher than that of muscle tissue and sebum + lipid,and the residual amount in the liver reached the highest(3424.8±73.3g/kg)at the 4th hour of discontinuation,and then began to decline,and the residue amount fell below the maximum residue limit in the 2nd day,and the 7th day was not detected;the typomycerin was rapidly eliminated in the muscle and sebum + lipid tissue,and the 8-α-hydroxy miaolin was not detected in the muscle and sebum + lipid in the 2nd.WT1.4 was used to calculate the telomeobacterium fumarate premix in liver,muscle,sebum + lipid periods of 2.45 days,2 days and 2days,respectively.It is recommended that when used according to the recommended dose of the tiamulin fumarate premix,the clinical recess period of chicken is 3d.3.The established 8-α-hydroxy miaolin residue detection method was used to evaluate the current status of taimyoxin residue in chicken meat and chicken liver in Henan marketUsing the determination method of 8-α-hydroxy miaolin of the residual marker of Taimiaoxin established in this study,295 batches of chicken meat and chicken liver samples taken from Jiudi City in Henan Province were tested.A total of 5 positive samples of 8-α-hydroxy Miaolin residue were detected in 295 samples,with a total detection rate of 1.7%.The detection rates of different types of samples were: chicken liver(2.9%)> chicken(1.3%);different links detection rates: farmers’ markets(2.3%)> slaughterhouses(1.5%)> supermarkets(1.3%).The residue of positive samples was: chicken meat 56.8~63.2μg/kg and chicken liver 128.3~176.5μg/kg,both lower than the maximum residue limit stipulated by the state.It shows that the safety risk of the residual marker 8-α-hydroxy miaolin in chicken meat and chicken liver sold in Henan Province is low,and it also proves that the established detection method can meet the actual sample detection needs.