Establishment And Optimization Of Tissue Culture System Of The Stems Of Acer Pseudosieboldianum

Acer pseudosieboldianum is a deciduous shrub or small tree of Acer in Aceraceae,with high ornamental value,economic value and ecological value.As a landscape tree species,Acer pseudosieboldianum is more and more popular for its beautiful tree-shaped,there is a growing development trend inmarket.At present,thepropagation of A.pseudosieboldianumismainlyby seed.However,seed propagation has the characteristic of dormancy,and this results in the lower reproduction efficiency.Propagation by grafting and cutting is easily affected by seasons,especially the technical difficulties of cutting propagation have not been broken through,which seriously restricts the development of A.pseudosieboldianum.Tissue culture rapid propagation technology not only has the advantages of sterility,high efficiency and rapidity,but also can improve the propagation rate of speed and survival.Therefore,it is of great practical significance to establish the tissue culture and rapid propagation system of A.pseudosieboldianum.In this study,we used A.pseudosieboldianum to screen out the best time for explants,the best disinfection method,the best basic medium,the ratio of plant growth regulators at each stage.The rapid propagation system of A.pseudosieboldianumhad been successfully established.Theresultsare summarizedas follows:1.The A.pseudosieboldianum explants were introduced as experimental materials.The best sampling time for explants was endly May,the explants of this period had high survival rate.The contamination rate was low,and the contamination rate was 13.33%;The appropriate disinfection method on axillary bud of A.pseudosieboldianum was 75%alcohol treatment for 40 s and 0.1%Hg Cl₂treatment for 8 min;The best combination of stem segment disinfection was 75%alcohol for30s and 0.1%Hg Cl₂for5 min.2.The optimal medium for axillary bud induction was MS+0.40 mg·L^-1IBA.The rate of axillary bud induction reached 93.33%;The optimal combination to multiplicate culture was MS+0.40 mg·L^-1IBA+0.80 mg·L^-1CPPU+0.60 mg·L^-16-BA,and the multiplication coefficient was3.87.3.The dark culture was not required when stem segments were induced.The optimum combination of callus induction from stem segment was MS+0.40 mg·L^-1IBA+0.70mg·L^-16-BA+0.20 mg·L^-1TDZ,with the highest induction rate as 69.44%;The optimum combination for callus multiplication was MS+0.50 mg·L^-16-BA+0.30 mg·L^-1IBA,and the multiplication coefficient was 2.90;The optimum combination for callus differentiation was MS+0.40 mg·L^-1TDZ+0.15 mg·L^-1NAA.The differentiation rate was 43.89%,and the average number ofdifferentiated budswas6.00.