Study On The Effect Of Tea Polyphenols On Acne And Skin Care And The Effect On Skin Flora

Objective:Tea Polyphenols(TP)is one of the main natural products in tea,which has anti-oxidation,anti-inflammation,antibacterial and other effects,and is widely used in medicine,health care products,food and other fields.However,the pharmacological effects of TP on cosmetics are rarely studied.To investigate the anti-acne effect of tea polyphenols(TPS)and its effect on acne skin flora,and to provide scientific basis for the development of natural and safe efficacy cosmetics.Methods:1.In vitro anti-acne experimental study of tea polyphenols(1)Antibacterial function studyMicrobroth dilution method and macrostatic zone method were used to measure MIC and inhibition zone size of tea polyphenols EGCG,TPP and commercial antibacterial reference products(p-hydroxyacetophenone,octanoyl glycerate ester,1,2-hexanediol)against the main pathogenic bacteria of acne,such as P.acnes,S.aureus,S.epidermidis,M.Furfur.(2)Oil control function researchThe lipid secretion model of human sebaceous gland cells SZ95 stimulated by oleic acid was established.The cells were stained with oil red dye and Nile red dye to detect the inhibitory effect of tea polyphenols,EGCG,TPP and commercial oil control standards(salicylic acid,zinc pyrrolidone carboxylic acid(PCA-zinc),and tea tree essential oil)on oleic acid stimulated lipid secretion of SZ95 cells.(3)Anti-inflammatory function researchHuman skin fibroblast HSF was stimulated by LPS to establish a model of cell inflammation and injury.ELISA kit was used to measure levels of IL-6,IL-8,IL-1β and TNF-α in LPS-stimulated HSF cells treated with tea polyphenols,EGCG,TPP and commercial anti-inflammatory standard products(dipotassium glycyrrhizinate,Portulaca oleracea extract and Calm Yang).2.In vivo anti-acne experimental study of tea polyphenols:C57 mice were randomly divided into control group,model group,0.05% TP group,0.1% TP group and 0.5% TP group,0.8% TP group,1.0 %TP group and 1.5 %TP group.The acne mouse model was established by injection of Staphylococcus aureus suspension into the back skin of the mice.The skin abscesses on the back of mice were observed.ELSA was used to detect the levels of inflammatory factors IL-6 and TNF-α,as well as oxidative indicators ROS and MDA in skin tissue of mice.q RT-PCR was used to detect the m RNA expressions of IKKβ,IKBα and NF-κB(P65)in skin tissue.Immunofluorescence was used to detect the protein expressions of IL-6 and macrophage marker(F4/80)in skin tissue.3.Effects of tea polyphenols on skin microbiota in mice with acneSterile cotton swabs were used to collect the back skin of mice with acne,and 16 S r DNA high-throughput sequencing technology was used to analyze the composition and abundance of skin microbiota in mice with acne,and to find out the characteristic flora of acne in mice and the effect of tea polyphenols on acne skin microbiota.Results:1.In vitro experiment results show that,(1)Antibacterial test resultsThe inhibitory effects of tea polyphenols,EGCG and TPP on P.acnes were T PP>EGCG>tea polyphenols.The antibacterial effects of tea polyphenols,EGCG,TP P and commercial antibacterial standard(p-hydroxyacetophenone,octanoate glycerol,1,2-hexanediol)on Staphylococcus aureus and Staphylococcus epidermoides were as follows: EGCG>TP>octanoate glycerol>TPP>p-hydroxyacetophenone>1,2-hexanediol.The antibacterial effects against Malassezia furfur were in the order of p-hydroxyac etophenone>glyceroctanoate>1,2-hexanediol>tea polyphenols.(2)Oil control test resultsTea polyphenols,EGCG,TPP and commercial oil control standard products(salicylic acid,PCA zinc,tea tree essential oil)could effectively inhibit the lipid secretion of SZ95 cells stimulated by oleic acid in a dose-dependent manner,and the inhibitory effect was EGCG> tea tree essential oil >TPP>PCA zinc > tea polyphenols.(3)Results of anti-inflammatory experimentsTea polyphenols,EGCG,TPP and commercial anti-inflammatory controls(dipotassium glycyrrhizinate,purslane extract,Calm Yang)inhibited LPS-induced production of inflammatory factors IL-6,IL-8,IL-1β,and TNF-α in HSF cells in a concentration-dependent manner.The inhibitory effect of TP on IL-6 in the supernatant of HSF cells was better than that of dipotassium glycyrrhizinate,and was equivalent to EGCG,purslane extract and Calm Yang.The inhibitory effect of TP on IL-1β in the supernatant of HSF cells was better than that of dipotassium glycyrrhizinate and Calm Yang,and was equivalent to EGCG and purslane extract.The inhibitory effect of TP on IL-8 in the supernatant of HSF cells was better than that of dipotassium glycyrrhizinate,comparable to that of purslane extract,but lower than that of EGCG and Calm Yang.The inhibitory effect of tea polyphenols on TNF-α in HSF cell supernatant was better than that of dipotassium glycyrrhizinate and purslane extract,and was comparable to EGCG and Calm Yang.TPP at a high concentration(100 μg/ml)had a significant inhibitory effect on IL-1β,IL-8 and TNF-α in the supernatant of HSF cells(P<0.05,P<0.001).2.The results of in vivo acne elimination experiments showed that,TP could significantly reduce the symptoms of acne caused by Staphylococcus aureus infection in mice,significantly inhibit the content of IL-6,TNF-α and MDA in the skin tissue of mice with acne(P<0.05,P<0.001),and had a good protective effect on inflammatory acne lesions caused by bacterial infection.Tea polyphenols significantly inhibited the protein expression of IL-6 and F4/80 in the skin tissue of acne mice(P<0.001),and significantly reduced the m RNA expression of IKKβ,IKBα and NF-κB(P65)in the skin tissue(P <0.01,P<0.001).3.The results of acne skin microbiota showed that,Tea polyphenols can improve the diversity and richness of skin microbiota in mice with acne,reduce the proportion of Staphylococcus,anaerobic Bacillus,Oligotrophomonas,and increase the proportion of Proteus,Aglybacterium,Streptococcus,so as to improve the disorder of skin microbiota in acne mice.Conclusions:Tea polyphenols have significant antibacterial,oil control and anti-inflammatory activities,which can significantly alleviate the symptoms of acne in mice,Reduce the content of IL-6 and other inflammatory factors in the skin of acne mice,and regulate the abundance of skin microbiota in acne mice.Therefore,tea polyphenols have good effects in inhibiting acne inflammatory response,regulating sebaceous gland secretion,and regulating acne skin microbiota.