Isolation Of Chemical Constituents From Aristolochia Manshuriensis Kom.and Asarum Caulescens Maxim.and Evaluation Of Insecticidal And Antibacterial Activity

Aristolochia manshuriensis Kom.is a plant of Aristolochia family,and its main composition is aristolochic acid compound.Asarum caulescens Maxim.is a plant of Aristolochia family,and its main chemical constituents exist in its rhizome.This research conducted a systematic study on the chemical components of Aristolochia manshuriensis Kom.and Asarum caulescens Maxim.Silica gel column chromatography,Sephadex LH-20gel column chromatography,recrystallization and thin layer chromatography were used for separation and purification.Combined with pop analysis techniques(¹H NMR,¹³C NMR,ESI-MS),the structures of the compounds were analyzed,and its agricultural activity was determined.8 compounds were isolated from the methanol extract of Aristolochia manshuriensis Kom.They wereβ-Sitosterol（X-1）,1-O-Heptatriacontanoyl glycerol（X-2）,Daucosterol（X-4）,Aristolochic acid I（X-5）,6-O-p-Coumaroylglucose（X-6）,Aristoloside（X-7）,Aristolochic acid IIIa（X-8）.Compound 1-O-Heptatriacontanoyl glycerol（X-2）was isolated from Aristolochia manshuriensis Kom.for the first time.The insecticidal activity and bacteriostatic activity of the above 8 compounds were determined.After 72 hours of treatment,the LC₅₀ value ofβ-Sitosterol（X-1）to the 3rd instar larvae of Aedes aegypti was48.073μg/m L.The EC₅₀ values of 1-O-Heptatriacontanoyl glycerol（X-2）,Aristolochic acid I（X-5）and 6-O-p-Coumaroylglucose（X-6）against Colletotrichum musae are 666.273μg/m L,194.082μg/m L,128.624μg/m L.Daucosterol（X-4）had the best bacteriostatic effect on Pyricularia oryae Cavara,and the EC₅₀ value was 2059.568μg/m L.7 compounds were isolated from the methanol extract of Asarum caulescens Maxim.They were Shizuka-acoradienol（Y-1）,Palmitic acid（Y-2）,β-Sitosterol（Y-3）,Atractylenolide III（Y-4）,Daucosterol（Y-5）,Shizukaol F（Y-6）,Sitosteryl oleate（Y-7）.Shizuka acoradienol（Y-1）,Shizukaol F（Y-6）,Sitosteryl oleate（Y-7）was isolated from Asarum caulescens Maxim.for the first time.The insecticidal activity and antifungal activity of the above seven compounds were determined.After 72 hours of treatment,the LC₅₀ values of Shizuka-acoradienol（Y-1）,Palmitic acid（Y-2）andβ-sitosterol（Y-3）to the 3rd instar larvae of Aedes aegypti were 190.263μg/m L and 145.512μg/m L,48.073μg/m L.Shizukaol F（Y-6）had the best antifungal activity on Colletotrichum musae with the EC₅₀ value was 334.086μg/m L.Daucosterol（Y-5）had the best antifungal activity on Pyricularia oryae Cavara with the EC₅₀value was 2059.568μg/m L.Shizuka-acoradienol（Y-1）had the best bacteriostatic effect on Botryodiplodia theobromae,and the EC₅₀ value was 44.572μg/m L.This study enriched the chemical constituents of Aristolochia manshuriensis Kom.and Asarum caulescens Maxim.And found that Aristolochia manshuriensis Kom.and Asarum caulescens Maxim.have good insecticidal and antifungal activity,which provides a theoretical and experimental basis for the future development of green,safe,efficient and harmless botanical pesticides.