Screen On Bioactivity And Study On Hypoglycemic,Antitumor Activity Ingredient Of Gynostemma Pentaphyllum

Gynostemma Pentaphyllum(Thumb.)Makion is kind of a perennial grass climbing vine belonging to Cucurbitace Gynostemma BL.,which is widely distributed in various provinces in China.Gynostemma is rich in gypenosides,flavonoids,polysaccharides and other bioactive components.The pharmacological pharmacodynamics studies confirmed that Gynostemma had antitumor,antioxidation,anti-lipid and antiatherosclerotic effects,as well as neuroprotection and hypoglycemic effects in recent years,which have aroused widespread concern.Four extracts of Gynostemma were obtained in this study.Different models,antioxidant,inhibiting nitrosation,hypoglycemic and antitumor properties were used to evaluate the activities of the four extracts.The results showed that the water extract of Gynostemma pentaphyllum was the best in antioxidant and hypoglycemic with IC50 value of 17.31±2.03 mg/mL on inhibiting α-glucosidase;the ethanol extract can significantly inhibit the nitrosation and the proliferation of hepatocellular carcinoma cells HepG2 and SMMC7721,with IC50 values were 115.84±10.49 μg/mL and 70.98±3.13 μg/mL,respectively.Secondly,macroporous resin column,reverse phase silica gel column Sephadex LH-20 filtration chromatography and silica gel thin layer chromatography,were used to separate and purify ethanol/water extract.MNT method was used as antitumor guidance with HepG2 cells as test cells.SFFr.F1 is antitumor active site from Gynostemma pentaphyllum,with a speculation that that SFFr.F1 had better antitumor effect may be based on the synergistic effect of compounds in SFFr.F1.Then,HPFr.C 14 from water extract was the best α-glucosidase inhibitor after screening,with the IC50 value was 5.88±0.89 mg/mL.HPLC analysis showed that the HPFr.C14 fraction contained four major compounds,named Compound 1-Compound 4.Single Compound 1-Compound 4 with high purity from HPFr.C14 were isolated by semipreparative HPLC,identified by modern spectroscopy as Vincetoxicoside A(Compound 1),Rutin(Compound 2),Kaempferol-3-O-glucose-7-Orhamnoside(Compound 3),and Nicotiflorine(Compound 4).Among them,Compound 1 and Compound 3 were isolated from Gynostemma for the first time.