Effects Of Overexpression Of Bacillus Subtilis AMEP412 Gene On Drought Tolerance In Arabidopsis Thalian

In the process of growth and development,plants are closely connected with the surrounding environment.In different environments,plants will make corresponding responses to avoid harm.The elicitor can stimulate the plant to produce defense response and has the effect of inducing and enhancing the plant disease resistance.AMEP protein is a novel protein activator found in Bacillus subtilis BU412,which can induce allergic reactions in plants.In addition to improving disease resistance,it has been found in previous studies to increase drought tolerance.Our team cloned the AMEP protein encoding gene AMEP412 and translated it into Arabidopsis thaliana.In this experiment,based on the previous work of the research group,transgenic Arabidopsis thaliana plants were screened,identified and bred,and transgenic Arabidopsis T₂ homozygous strains were obtained for subsequent experiments.The results showed that the seed germination rate,seedling survival rate,root length and fresh weight of transgenic AMEP412 were significantly higher than those of wild Arabidopsis Thaliana,indicating that overexpression of AMEP412 gene had positive regulation on plant growth under drought stress.The physiological indexes of transgenic Arabidopsis thaliana and wild-type Arabidopsis thaliana were determined and their data were analyzed after 0 d,7 d,14 d and 5 d rehydration.The results showed that chlorophyll content,soluble sugar content and peroxidase activity of transgenic AMEP412 were significantly higher than those of wild-type Arabidopsis thaliana,while malondialdehyde content was significantly lower than that of wild-type Arabidopsis thaliana.These results indicated that overexpression of AMEP412 gene could effectively reduce the damage to plants under drought stress.In this experiment,the effects of drought stress on the transgenic plants were studied,the morphological and physiological indexes were determined and the data were analyzed,which laid a foundation for further elucidate the effects of AMEP protein on the drought tolerance of plants,and provided relevant experimental materials and theoretical basis for further research on the interaction between plants and microorganisms.