Establishment Of Prognostic Model Of Ovarian Cancer Based On Comprehensive Bioinformatics And The Molecular Mechanism Of NOTCH2NLA Mediating Ovarian Cancer Progression Through Epithelial Mesenchymal Transformation

Objective: In this study,the differentially expressed genes that may affect the prognosis of ovarian cancer were explored and established to a prognostic gene model through integrated bioinformatics.Cell experiments were conducted to verify the effect of NOTCH2 NLA expression on the biological behavior of A2780-GFP cells and explore the potential molecular mechanism of NOTCH2 NLA mediating the progression of OC,thus providing a new biological theoretical basis for targeted therapy and prognosis evaluation of OC.Methods: 1.The Genotype-Tissue Expression(GTEx)project and The Cancer Genome Atlas(TCGA)project were used to obtain m RNA sequencing datasets and clinical information to identify differentially expressed genes and the prognostic model of OC was constructed by survival analysis.2.Cell proliferation,wound healing,and transwell assays validated the effects of NOTCH2 NLA on the biological behavior in the A2780-GFP cell line.3.Western blot assay was performed to detect the effect of NOTCH2 NLA on the expression levels of E-cadherin,Vimentin and SNAI2(EMT-associated proteins).Results:1.Prognostic analysis of OC showed that PI3,CCDC80,IL27 RA,HERC1,NOTCH2 NLA were adverse prognostic factors,and HMGB3,CXCL11,and CXCL9 were protective prognostic factors.2.Cell assays confirmed that repression of NOTCH2 NLA could block the proliferation,migration,and invasion of A2780-GFP cells.3.Western blot assay confirmed that the expression of NOTCH2 NLA in OC was consistent with E-cadherin,but contrary to Vimentin and SNAI2.Conclusion: 1.The 8-gene model constructed by bioinformatics can effectively evaluate the prognosis of OC.2.Silencing NOTCH2 NLA inhibits the malignant biological behavior of A2780-GFP cells.3.The regulation of NOTCH2 NLA on EMT-related proteins is a key factor in the progression of OC.