Novel Potential Biomarkers Associated With Epithelial To Mesenchymal Transition And Bladder Cancer Prognosis Identified By Integrated Bioinformatic Analysis

Bladder cancer(BC)is one of the most common malignancies in terms of incidence and recurrence worldwide.The aim of this study was to identify novel prognostic biomarkers related to BC progression utilizing weighted gene co-expression network analysis(WGCNA)and further bioinformatic analysis.First,we constructed a co-expression network by using WGCNA among 274 TCGA-BLCA patients and preliminarily screened out 4 genes(CORO1C,TMPRSS4,PIK3C2 B and ZNF692)associated with advanced clinical traits.In support,GSE19915 and specimens from 124 patients were used to validate the genes selected by WGCNA then CORO1 C and TMPRSS4 were confirmed as hub genes with strong prognostic values in BC.Moreover,the result of gene set enrichment analysis(GSEA)and gene set variation analysis(GSVA)indicated that CORO1 C and TMPRSS4 might be involved in the process of epithelial to mesenchymal transition(EMT)reversely.In addition,high expression of CORO1 C was found to be significantly correlated with tumor-infiltrating neutrophils(TINs),a negative regulatory component which facilitated tumor distant progression and induced poor clinical outcome.In conclusion,our study first identified CORO1 C and TMPRSS4 as vital regulators in the process of tumor progression through influencing EMT and could be developed to effective prognostic and therapeutic targets in future BC treatment.