Antibacterial Properties Of Bimetallic Compound Nanozymes

Bacterial infection is a worldwide problem threatening human health,and also a major challenge in the field of scientific research.Because of its excellent catalytic activity and physicochemical properties,nanozymes are regarded as the next generation of antibiotics.However,due to its relatively low catalytic efficiency,biocompatibility and limited antibacterial effect,its application potential is seriously limited.Herein,in order to explore more biosafety and efficient nanozyme antibacterial materials,ferrous molybdate(FeMoO₄)and chalcopyrite(CuFeS₂)nanomaterials with excellent peroxidase catalytic activity were prepared by a simple solvothermal method,and their antibacterial properties and application potential in the treatment of wound infection were studied.1.In order to develop biocompatible and efficient nanozyme antibacterial materials,we prepared ferrous molybdate(FeMoO₄)nanomaterials with excellent peroxidase(POD)catalytic activity by one-step solvothermal method.The as-prepared FeMoO₄ nanomaterials are composed of Fe(II)and molybdate(MoO₄^2-),which is beneficial to the enhancement of the catalytic activity of FeMoO₄ nanozymes.Through the study of enzyme kinetics,compared with the natural horseradish peroxidase(HRP)and other peroxidase mimics,the prepared FeMoO₄has lower Km value and higher Vmax,which means the higher affinity and catalytic efficiency of FeMoO₄ peroxidase to substrate.In the presence of low concentration of H₂O₂,the FeMoO₄nanoenzyme showed high antibacterial ability and could effectively kill E.coli,a gram negative bacterium.The results show that the FeMoO₄ nanoenzyme mediated antibacterial system possess excellent antibacterial ability,can effectively inhibit wound infection and promote wound healing.2.Although peroxidase mediated chemokinetic therapy(CDT)can effectively resist bacteria,factors such as narrow antibacterial spectrum,single treatment mode and high dosage of drugs seriously limit the antibacterial effect.In the interests of development of biosafety,high efficiency and broad-spectrum antibacterial ability of nanozyme antibacterial system,CuFeS₂ nanoparticles(NPs)with photothermal effect and peroxidase catalytic activity were used to construct a combined antibacterial system of photothermal and chemokinetic therapy.CuFeS₂ NPs could break the antioxidant balance by consuming intracellular glutathione(GSH),which is more conducive to the production of oxidative stress.The results of in vitro antibacterial experiment showed that the proposed antibacterial system achieve more than 99%inactivation efficiency of methicillin-resistant Staphylococcus aureus(MRSA),hyperspectral bacteria?-Escherichia coli(ESBL)and Pseudomonas aeruginosa(PA)even at low concentration(2?g/m L).In vivo experiments further show that the antibacterial system with excellent antibacterial ability can effectively treat the wound infected by methicillin-resistant Staphylococcus aureus(MRSA)and promote the wound healing.This study shows that the excellent antibacterial ability and good biocompatibility make CuFeS₂ NPs become a potential anti-infection nanozyme with broad application prospects.