| Objective: To evaluate the in vitro antibacterial properties of the iodine-doped Ti O2 coated PVC endotracheal tube material.Methods: Took the side length of 20mm×20mm(± 2mm)×2 mm standard medical PVC endotracheal tube material samples and specifications of the same new iodine-doped Ti O2 coated PVC antibacterial endotracheal tube material samples each 18 piece, based on light irradiation, they are divided into four groups: the a N group(n=9), the standard medical PVC endotracheal tube material sample + without light processing; a L group(n=9), the standard medical PVC endotracheal tube material sample + visible light irradiation treatment; b N group(n=9), the new antibacterial trachea iodine doped Ti O2 plating film of PVC material sample + without light; BL group(n=9), new iodine-doped Ti O2 coated PVC antibiosis endotracheal tube antibacterial endotracheal tube material PVC samples + visible light irradiation. Each sample surface inoculated with 0.05 ml concentration of 1x108 cfu/ml bacteria liquid concentration gradient. Put a L group and b L group at greater than 400 nm wavelength and light intensity of 500 mw/cm2 light conditions to develop; Will the a N group and b N group training under dark condition. After all the samples in 2h elution bacteria count, test the antibacterial performance of samples material to pseudomonas aeruginosa, staphylococcus aureus and escherichia coli. Take the other specifications of the standard medical PVC endotracheal tube material sample and new iodine-doped Ti O2 coated PVC antibacterial endotracheal tube materials samples each 3 pieces, annotation for the c L and d L group respectively, the two materials were soaked in a concentration of 1 x 108 cfu/ml of pseudomonas aeruginosa bacteria liquid plate, at more than 400 nm wavelength, and light intensity 500 mw/cm2 lighting condition, after 48 h,scanning electron microscope observe bacterial growth on surface.Results: Compared with the a N group, a L group and b N group, b L group iodine-doped Ti O2 coated PVC endotracheal tube material under the condition of irradiation significantly reduced the number of living bacterium on its surface.(P<0.05). Scanning electron microscopy(SEM) showed that under the condition of light d L group of iodine-doped Ti O2 coated PVC endotracheal tube material surface plating adhesion of bacteria decreased significantly in the c L group standard medical PVC endotracheal tube material.Conclusion: The visible of light activated iodine-doped Ti O2 coated PVC endotracheal tube material has obvious antibacterial activity.Objective: To observe influence of the post visible light activated antibacterial trachea iodine-doped Ti O2 coated endotracheal tube miniature pig’s airway and lung tissue bacterial burden.Methods: 24 healthy adult small Bama suckling pig, 21.7±1.2 kg, male and female each half and half. According to the mechanical ventilation when insert the endotracheal tube is treated with coating and whether the introduction of light during mechanical ventilation, animals were randomly divided into 4 groups: AN group(n=6), insert the PVC endotracheal tube without coating processing standard, without introducing light; AL group(n=6), the PVC endotracheal tube without coating processing standard + introduction of visible light(light intensity: 0.2mw/cm2); BN group(n=6), iodine-doped Ti O2 coated PVC endotracheal tube, without introducing light; BL group(n=6), iodine-doped Ti O2 coated PVC endotracheal tube + introduction of visible light(light intensity: 0.2mw/cm2). All the animals after anesthesia via tone line mechanical ventilation tube insertion, 1h after mechanical ventilation and 8 h respectively two points within the animal oral vaccination with good concentration in advance about concentration in 1×107 cfu/ml of Pseudomonas aeruginosa bacteria liquid 5ml. Mechanical ventilation after 72 h excessive anesthesia to put animals to death, anatomical materials strict aseptic conditions. To quantify the endotracheal tube surface discharge score; Trachea, bronchi and lung parenchyma tissue samples and the endotracheal tube samples respectively after homogenate grinding or supersonic elution treatment to microbiology laboratory bacteria qualitative quantitative analysis; Optical microscope observation of airway and lung tissue and proceed pathological changes, and quantitative score compared to the injury of lung tissue; electronmicroscope scanning(SEM) to observe the bacterial biofilm formation on the surface of the endotracheal tube.Results: Compared with the three groups of AN, AL and BN, group of BL animal secretion on the surface of the trachea score significantly decreased(P < 0.05); Electron microscopy scanning(SEM) shows that BL catheter surface bacterial burden and bacterial biofilm decreased significantly than the AL group; Compared with the three groups of AN, AL, and BN, group of BL animal trachea, lung tissues and pipe surface engraftment of pseudomonas aeruginosa and the total aerobic bacteria reduce significantly(P < 0.05). Compared with the three groups of AN, AL and BN, group of BL animal lung injury score decreased significantly(P < 0.05).Conclusion: Visible light activated iodine-doped Ti O2 coated endotracheal PVC tube endotracheal tube can reduce the animal mechanical ventilation endotracheal tube surface, airway and lung tissue aerobic bacteria such as pseudomonas aeruginosa engraftment. |
PDF Full Text Request