| Objective:Using metabonomics technology to explore the molecular mechanism of electroacupuncture at the acupoints of the spleen and stomach meridian on acute gastrointestinal mucosal injury and its repair.Through the analysis of differential metabolites and metabolic pathways,the molecular mechanism of electroacupuncture in the treatment of gastrointestinal mucosal injury and its The clinical treatment provides some experimental data support.Methods:According to the random number table method,24 SPF male mice were divided into four groups: normal group(group C),model group(group M),Zusanli group(group Z),and Sanyinjiao group(group S),each with 6 mice.After one week of adaptive feeding,in addition to the normal group,the model group,the Zusanli group,and the Sanyinjiao group all used restraint cold soaking method to induce acute gastrointestinal mucosal injury models according to the experimental protocol.After successful modeling,the Zusanli point group and Sanyinjiao point group were treated with electroacupuncture.After the intervention,observe and record the behavioral characteristics of the mice,take the gastric tissue and duodenal tissue for macroscopic observation and histopathological observation under the light microscope,and use the hydrogen nuclear magnetic resonance spectroscopy(1H-NMR)to detect the mouse gastric tissue.The changes of metabolites in duodenal tissue before and after intervention,the use of pattern recognition analysis to screen metabolic markers.Metabo Analyst was used to analyze the metabolic pathways of the obtained metabolic markers.Result:1.Observation of mouse behavior:(1)The number of standing upright: Compared with the normal group,the number of standing upright was significantly increased in the model group(p<0.05);compared with the model group,the number of standing upright in the Zusanli group was significantly increased.Decrease(p<0.05),but did not reach the upright level of the normal group;compared with the model group,the number of upright times of the Sanyinjiao mice increased,and maintained the same trend as the number of upright mice in the Zusanli group.(2)Fecal pellets:Compared with the normal group,the fecal pellets of the model group increased significantly(p<0.05);compared with the model group,the fecal pellets of the Zusanli group were significantly reduced(p<0.05);compared with the model group In comparison,the fecal pellets of mice in the Sanyinjiao group also decreased significantly(p<0.05).? 3 Mine field experiment: Compared with the normal group of mice,the percentage of time in the central area and the percentage of distance to the central area of ??the model group showed a significant increase(p<0.05);compared with the model group of mice Compared with mice in the Zusanli group,the percentage of time in the central area and the percentage of distance in the central area were significantly reduced(p <0.05);compared with the model group,the percentage of time in the central area and the percentage of distance in the central area of??the Sanyinjiao group were different.decline.(4)Swimming:Compared with the mice in the normal group,the inactive time of the model group mice in the water,that is,the resting time was significantly reduced(p<0.05);compared with the model group mice,the Zusanli group mice were still in the water The time was significantly increased(p<0.05);compared with the model group,the water resting time of the Sanyinjiao group also showed an increasing trend.2.Observation of the tissue morphology of the mouse digestive tract:(1)Stomach tissue: The surface of the stomach tissue can be seen with different degrees of punctate and linear hyperemia.Under light microscope,the gastric mucosal epithelial structure of mice in the normal group is complete,the gland ducts are arranged neatly,and there are no inflammatory cells in the submucosa and muscle layer;the gastric mucosa of the model group has severe epithelial cell necrosis and exfoliation,disordered gland duct arrangement,and blurred nuclei,Capillary hemorrhage,myometrial inflammatory cell infiltration and even mucosal cavities;the gastric mucosal ducts of the Zusanli group are basically neatly arranged,the mucosal cells are loosely arranged,capillary hyperplasia,and some inflammatory cells are still visible in the submucosa;mice in the Sanyinjiao group Although the gastric mucosa has improved to a certain extent,the epithelial structure is still incomplete,the arrangement of glands is disordered,and the intercellular substance infiltrates inflammatory cells.(2)Duodenum: The duodenal mucosal epithelial structure of mice in the normal group is complete and clear,the ducts are arranged neatly,without bleeding and inflammatory cells;the ducts in the model group are arranged disorderly,the epithelial structure is peeled off,and the interstitium is filled with a large number of inflammatory cells,Cell edema,capillary hemorrhage;The condition of the duodenal mucosa of the mice in the Zusanli point group was significantly improved,the ducts were arranged neatly,the interstitial inflammatory cells were significantly reduced,and the intracellular edema was reduced;the duodenum of the mice in the Sanyinjiao point group The mucosal ducts are basically neatly arranged,the cytoplasmic nucleus is basically clear,there are still epithelial cells peeled off,interstitial inflammatory cells,and although there is capillary hyperplasia,there is still bleeding.3.Mouse stomach and duodenal metabolomics: 1H-NMR results showed that 15 different metabolites were found in mouse stomach and duodenal tissues including: betaine,choline,alanine,phosphoric acid Choline,ethanolamine,serine,adenosine,inosine,lactic acid,glycine,pyruvate,glycerophosphocholine,glutamine,and ?-glucose glutathione include a total of 12 metabolic pathways.Conclusion:1.Electroacupuncture at Zusanli has obvious repairing effect on gastric mucosal injury and duodenal mucosal injury.At the same time,it is found that electroacupuncture at Sanyinjiao point also has a certain degree of repairing effect on gastric mucosal injury and duodenal mucosal injury.2.Based on 1H-NMR metabolomics technology and multivariate data analysis,it shows that Zusanli and Sanyinjiao can promote the repair of acute gastric mucosal injury in mice.By promoting the release and metabolism of local tissue energy,and enhance cell metabolism and activity,antioxidant capacity,and then repair damaged gastrointestinal mucosal tissue. |
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