Extraction Of Active Ingredients From Cat's Claw And Development Of Dispersible Tablets

The infection rate and fatality rate of tuberculosis have been high every year in the world,and the number of patients infected with tuberculosis in our country is the second in the world.Polysaccharide is a kind of immunomodulator,which has the characteristics of low cytotoxicity,and the content of polysaccharide in cat claw grass is high,which can regulate the immune function of the body,and cat claw grass has a better anti-tuberculosis effect because of its immune regulation function.The study confirmed that the alcohol extract of Radix Ranunculi Ternati could significantly inhibit mycobacterium tuberculosis,the toxicity was lower than isoniazid,rifampicin,and was nontoxic at a certain dose.Radix Ranunculi Ternati is currently the only listed anti-tuberculosis Chinese medicine.At present,the single preparation of Radix Ranunculi Ternati has been listed as cat claw grass capsule(ministerial standard: WS3-B-2035-95),into extract and mix with fine powder,then use water to make pellets and then pack capsules;cat claw grass pills(Henan province medicine standard :1944),the preparation method is to fry some fine powder water of cat claw grass into paste,dry and crush fine powder,combine fine powder,water flooding made of sugar coated pills.In both dosage forms,the active components are unclear and the preparation process is simple and rough.Therefore,it is a problem to be solved in this study to enrich the active components of the herb and improve the deficiency of the original clinical dosage form.The optimum ethanol extraction process,polysaccharide extraction process and refining process were optimized by orthogonal experiment design and single factor investigation.After concentrated,dried,crushed and sifted,ethanol extraction powder and polysaccharide powder were obtained.In this paper,the quality standard of the three batches of Radix Ranunculi Ternati chinensis dispersible tablets was established,and the stability of Radix Ranunculi Ternati chinensis dispersible tablets was studied by accelerated test and long-term test.The study shows that the best alcohol extraction technology of Claw is:75% ethanol was refluxed for 2 hours each time for 2 hours each time,the best extraction technology of polysaccharides from Radix Ranunculi Ternati was 12 times water for 3 times each time for 3hours;the best refining technology of crude polysaccharides was separation by membrane with 60 k D and 20 k D molecular weight.Ultrafiltration under conditions MPa 20?30?,0.10 and 0.15;The preparation forming technology of Radix Ranunculi Ternati dispersible tablet is:filling agent is microcrystalline cellulose,disintegrating agent is PVPP : LS-HPC(2 : 1),added by PVPP and SDS,Lubricant: silica gel with powder,the best weight is 1.0 g,,the finished product of dispersible tablet is obtained.The dispersion sheet has moderate hardness and disintegration time is less than 3 minutes.The indexes of accelerated stability test and long-term stability test of Radix Ranunculi Ternati dispersible tablets are in accordance with the regulations,and the stability of drugs is better.The preparation process of the dispersible tablets is feasible and the quality of the dispersible tablets is stable.