Research On Drug Quality Control System Of Yinhe Prescription

Yinhefang is the clinical experience of Academician,Wang Yongyan,The prescription composed of Panax ginseng,Panax notoginseng and ginkgo leaf,has a positive effect with Supplementing qi and promoting blood circulation and dredging collaterals,attending coronary heart disease,The heart qi deficiency and blood stasis syndrome.Our preliminary established the extraction process and enrichment of preparation process of Yinhefang.And efficacy validation showed that the enrichment,alcohol extraction,aqueous extraction of Yinhefang have the effect of invigorate the circulation of blood and hemostasis.Among them,the effect of enrichment is especially significant.By thrombus and anticoagulant effect model,the mixed in different proportions verifies the effectiveness of richment,grope for efficacy of the best combination ratio,and through the pharmacy-effect relation analysis,found that each component content ratio changes and the relationship with the effect,it provides idea and method for new drug development.Chaper 1The research progress on chemical components and pharmacological activities of Yinhefang were reviewed to provide a reference for the further research and development.chaper 2Established a quality control method for preparations of Yinhefang.Method for the determination of total phenol,total terpene,amino acids,total alkaloids,total lactones in the preparations and the herbs of prescription was established by using spectrophotometry;Method for the determination of pr,gg,gr,gb,qn,kl,in,ne in preparations and the herbs of prescription was established by using HPLC.Established the method of the HPLC characteristic spectrum quality control of the preparations and the herbs of prescription.Chpater 3Determination of total composition and target components in the preparations;HPLC characteristic spectrum of enriched ? should be five characteristic peaks,qn for reference,which peaks and reference peaks retention time was the same,and qn with reference to the corresponding peak to S peak;HPLC characteristic spectrum ? of enriched ? should be 25 characteristic peaks,pr,gg,gr,gb for reference,which four peaks and reference peaks retention time was the same,and gg with reference to the corresponding peak to S peak;HPLC characteristic spectrum ? of enriched ? should be 9 characteristic peaks,qn,k1,in for reference,which three peaks and reference peaks retention time was the same,and qn with reference to the corresponding peak to S peak.Chapter 4Determination of total composition and target components in the herbs of prescription,combined with the correlation analysis,better quality herbs can be screened.Determine the HPLC characteristic spectrum of the herbs of prescription,HPLC characteristic spectrum of Panax notoginseng should be nine characteristic peaks,quercetin,kaempferol,isorhamnetin for reference,which three peaks and reference peaks retention time was the same,and quercetin with reference to the corresponding peak to S peak;HPLC characteristic spectrum of Ginkgo biloba.should be eleven characteristic peaks,quercetin,kaempferol,isorhamnetin for reference,which three peaks and reference peaks retention time was the same,and quercetin with reference to the corresponding peak to S peak;HPLC characteristic spectrum of Nelumbo nucifera Gaertn.should be seven characteristic peaks,Hyperoside,Isoquercitrin and nuciferine as reference,which three peaks and the reference peak retention time of the same,and nuciferine reference peaks corresponding to the peak to S peak.Chapter 5Use thrombosis and anticoagulation pharmacodynamic models to plasma TXB2,6-ketoPGF1a,prothrombin PTHR and thrombin THR content as biochemical indexes of different proportions of concentrate ratio pharmacodynamic verification,explore the best combination of compatibility,through the medicine-efficacy of characterization of correlation analysis,to explore the access of preparing product with the best effect.Thrombus model efficacy best is B.Anticoagulant model best is F.Thrombosis model in the preferred composition of anticoagulant efficacy of the components having different proportions,indicating the changes of the ingredients proportion have a great impact on the pharmacodynamics.Chapter 6To summarized the full text.