Application Study Of Functional Electrospun Wound Dressings

With the rapid development of science and technology as well as the transformation of a large number of laboratory achievements,emerging nanomaterials have begun to influence people’s daily life more and more deeply.Electrospinning technology as the simplest and most efficient method to prepare nanomaterials in large quantities are widely spread throughout the world.Meanwhile,nanofiber membrane as one of the end product is different from other material of nanometer scale effect,huge specific surface area,porosity,in terms of composition and structure,and also can be design highly,these advantages make the electrospinning fiber membrane has broad prospect of application in biomedical research,especially in the bleeding,tissue engineering,drug slow release,nerve repair and wound dressings,etc.At present,electrospinning fiber membrane as wound dressing still has three shortcomings as follow:(1)Two-dimensional fiber membrane is difficult to fully meet the stringent requirements of wound dressing;(2)Electrospinning films with antibacterial effect are often only doped with antibiotic drugs,and have a weak effect on drug-resistant superbacteria;(3)The large size of the electrospinning equipment used in the laboratory and its heavy dependence on mains electricity greatly limits its applicability in handling outdoor medical accidents.Based on the above problems,we have done some research from the reasonable design of electrospinning film and the improvement of existing electrospinning equipment.Firstly,we designed wound dressing with a sandwich structure that balances antimicrobial activity and biocompatibility while promoting wound healing.This mainly depends on the incorporation of silver nanoparticles(Ag NPs)into the electrospun nanofibers and the rational design of the sandwich structure used for dressing.The bottom layer of the fiber membrane is a hydrophilic nanofiber made of PCL and PCL/Gel,and the top layer is made of hydrophobic PCL nanofiber.The Ag NPs loaded PCL/Gel nanofibers were sandwiched between the two layers.Compared with the commercial silver sulfadiazine dressings(SSD,Urgotul,France),this wound dressing has shown competitive antibacterial properties,lower cytotoxicity,and accelerated wound healing in the event of skin injury in mice.In summary,by balancing the biocompatibility of the electrospun nanofibers with the broad-spectrum antimicrobial activity of Ag NPs,we propose a novel multifunctional wound dressing suitable for effective wound healing and related applications.Secondly,we developed a portable outdoor electrospinning device(150g weight)that can be used for green synthesis of Cu S NPS composite nanofibers,which can be deposited in situ at the wound surface,while enabling rapid outdoor hemostasis and superbacteria ablation.These in-situ electrospun nanofiber membranes have better densification on rough wound surfaces,so they not only accelerate hemostasis(< 6 s),but also shorten the healing time of superbug infected wounds(18 days).This bifunctional nanofiber,combined with portable electrospinning devices,can be used for rapid outdoor hemostasis and simultaneous ablation of superbacteria,greatly facilitating the development of outdoor first aid.