Preparation And Antibacterial Application Of Bio-based Electrospinning Fibrous Membrane

Electrospinning technology can simply and efficiently prepare large quantities of nanomaterials.With its advantages of good nano scale effect,huge specific surface area porosity and permeability,it can eliminate excess wound exudate and improve wound healing efficiency Moreover,electrostatic spinning technology has a high degree of controllability in terms of composition and structure,which can provide an antibacterial environment suitable for wound healing and also play a protective role.These advantages make electrospinning fibrous membrane have a broad application prospect in wound dressing researchThe Electrospinning fibrous membrane as a wound dressing still exist the following flaws:1)nanoparticles(biological active ingredients and inorganic antibacterial agent,antioxidant,metal)dispersed in the spinning solution.Although the electrospinning technology was simple,the nanoparticles was wrapped in fiber tend to use efficiency is not high,it is difficult to fully meet the strict requirements of dressing;2)A large number of antibiotics and quaternary ammonium salts are easy to develop drug-resistant superbacteria and make the antibacterial performance of functional fibrous membrane ineffective;3)Microorganisms tend to adhere to the surface of biological materials,leading to the formation of difficult to eliminate biofilm and increasing the risk of bacterial infection.In view of the above problems,this paper starts from the reasonable design of electrospinning membranes,and aims to build multifunction composite nanomaterials,so as to better play the antibacterial role of functional nanoparticles.It is expected to be applied to biological materials to overcome medical problems and ensure human health and safety.The specific research contents of this paper are as follows:1.PLA/CAP/TH multi-scale composite fibrous membrane was prepared by blending with tetracycline(TH)and polylactic acid(PLA)and cellulose phthalate acetate(CAP)as matrix materials.Under the condition of adding tetracycline hydrochloride,SEM showed that the fiber diameter in the electrospinning membrane presented a Gauss distribution.The diameter is about 3-5 μm,and with the increase of CAP concentration,the average fiber diameter changes little,and the fiber morphology remains uniform and smooth.The morphology and properties of fibrous membranes were characterized by XRD,TG and mechanical properties.Finally,through antibacterial and zebrafish experiment,it was verified that the fibrous membrane had good biocompatibility and excellent bacteriostatic performance.The antibacterial activity results showed that the antibacterial diameter of PLA/CAP/TH fiber membrane was 18-35 mm.The water contact angle of composite fiber membrane reaches 120°,which is consistent with that of pure polylactic acid nanofiber membrane,providing hydrophobic material for the construction of single guided water fiber membrane.The above tests proved that the composite fibrous membrane can be used for further study of wound dressing.2.A micro-nano structure fibrous membrane doped with electrospun silver nanoparticles was designed,and then a bio-based PPAT-(TA/QC)n nanofiber pad was prepared by layer-by-layer self-assembly technology to be used as a multi-functional antibacterial wound dressing.The material has good flexibility,antibacterial activity,good blood compatibility and good scavenging ability of reactive oxygen species.The contact Angle test shows that the surface of the self-assembly structure has good wettability and hydrophilicity.In addition,the antibacterial experiments show that the prepared membrane has effective broad-spectrum antibacterial ability and can inhibit the infection of model microorganisms.Finally,antioxidant properties provide an excellent prospect for the formation of physiological for wound healing.Therefore,this work lays a foundation for the construction of a friendly,non-toxic,antibiotic-free biofilm as a multifunctional biomedical antibacterial skin dressing.At the same time,a large amount of tannic acid was used to reduce the silver ions to produce silver nanoparticles on the surface of the fiber,and the surface layers of quaternylated chitin were self-assembled to improve the antibacterial effect.Compared with the situation of directly acting on the wound surface,the cytotoxicity of silver nanoparticles was significantly reduced.The structural design of nanofiber dressing greatly improves the function of dressing.3.A kind of organic composite nanofiber membrane fibrous membrane(PCTCP)with high strength,high elasticity,self-cleaning,anti-pollution,super hydrophobicity and low toxicity was prepared by using bio-based polymer materials cellulose(CNC)and polyurethane(PU)as raw materials through electrostatic spinning technology and blending technology.The ultra-low toxicity of PCTCP membrane and its good biocompatibility make it possible to apply composite fibrous membrane in vivo,such as wound dressings and public health products.On the basis of the introduction of photothermal conversion materials,the fiber membrane is sensitively responsive to ultraviolet and visible light by loading titanium dioxide,so that the composite nanofibers can kill bacteria by photodynamic and photothermal methods,which provides research ideas and scientific guidance for the study of new wound dressing system.