Association Study Of NNMT And FGF21 Gene Polymorphisms And Individual Differences In Health Promotion Effects Of HIIT

Objective: Exercise is good for health.High-intensity interval training(HIIT)is one of the most recommended fitness methods in recent years.It has a positive effect on skeletal muscle metabolism and cardiovascular regulation,and its effect will not be lower than that of lowto-medium intensity.Exercise,even in terms of increasing the maximum oxygen uptake(VO2max),HIIT is more time-sensitive than continuous low-intensity aerobic exercise.And because the training time is short,the discomfort symptoms can be avoided through intervals,so it is easier to accept and complete the training.However,the effect of exercise on the promotion of health has large individual differences,and genetic effects are one of the important influencing factors.Therefore,this study intends to explore the relationship between NNMT and FGF21 gene polymorphisms and individual differences in the health promotion effects of HIIT,so as to provide references for formulating individual precise exercise intervention programs.Methods: Four universities,Inner Mongolia Normal University,Anqing Normal University,Jiangxi Normal University,and Lanzhou City University organized HIIT training intervention.A total of 220(male=100,female=120)subjects completed the 12-week HIIT training program.Test their body shape,body composition,aerobic endurance and other indicators before and after the intervention.The effect of high-intensity interval training is expressed by the amount of change of each index before and after training.The Illumina CGA chip was used to type the four tagged SNPs of two genes,NNMT rs694539,NNMT rs1941404,FGF2 rs2071699,and FGF2 rs11665841.The chi-square test was used to calculate whether the distribution of each genotype conformed to the Hardy-Weinberg genetic balance.The changes of different physiological phenotypes of subjects before and after endurance training used paired sample t test,and the difference of physiological phenotypes between different genotypes used covariance analysis.Regression analysis of the contribution of positive association sites to the impact of indicators.Results: 1)HIIT health promotion effect and individual differences: After 12 weeks of HIIT,body shape,body composition and aerobic exercise endurance indicators have significantly improved,but there are large individual differences.(1)Males: The skeletal muscle mass increased significantly by 0.355kg(variation range:-3.0--3.60 kg,p < 0.01).Body fat mass was significantly reduced by-1.174kg(variation range was-9.60--2.60 kg,p<0.01).VO2 max increased significantly by 0.300L/min(variation range:-0.45--1.47L/min,p<0.01).(2)Females: The skeletal muscle mass increased significantly by 0.592kg(variation range:-1.60-7.30 kg,P<0.01).Body fat mass is significantly reduced-0.228kg(-4.80--5.50 kg,P<0.05,whether there is a significant change,it is not mentioned in the article).VO2 max increased significantly by 0.186L/min(variation range:-0.380--0.700L/min,p <0.01).2)The correlation between genetic polymorphism and the health promotion effect of HIIT:(1)Males: The body fat training effect is significantly associated with NNMT rs694539.The training effect of CT genotype carriers(-1.518kg)is significantly higher than that of CC genotype(-0.974kg)and TT genotype(-0.233kg).The frequency of CT genotype is 49%;the skeletal muscle quality training effect is significantly correlated with NNMT rs1941404,the AG genotype(0.591kg)is significantly higher than the AA genotype(0.363kg)and GG genotype(-0.080kg),and the AG genotype frequency 45%.(2)Females: Body fat training effect is significantly related to NNMT rs1941404,GG genotype training effect(-0.080kg)is significantly higher than AA genotype(-0.041kg)and AG genotype(0.014kg),GG genotype frequency is 25%.The results of stepwise regression analysis found that this locus can explain 5.1% of the difference in body fat changes.No significant association between FGF21 gene polymorphism and HIIT training effect was found.Conclusion: NNMT gene polymorphism is associated with the individual difference effect of HIIT health promotion.1)rs694539 can predict the training effect of male body fat,CT is the dominant genotype,and carriers have better intervention effects;2)rs1941404 can predict the training effect of male skeletal muscles.AG is the dominant genotype,and carriers have better intervention effects.3)rs1941404 can predict the training effect of girls’ body fat,GG is the dominant genotype,and carriers have better intervention effects.And this site affects 5.1% of the individual difference in the effect of body fat intervention.