| Purpose: High-fat diet is one of the common factors that cause the body energy imbalance,and then obesity occurs.Exercise can induce white adipose tissue browning,promote heat production in adipose tissue,and increase energy expenditure.But the mechanism is not clear.PGC-1α,PPARγ and PRDM16 are involved in regulating fat metabolism.They are also important regulators of white adipose tissue browning.In this research,will observe high-fat diet and exercise effecting on expression and interaction of PGC-1α,PPARγ and PRDM16 to explore the movement regulation of the white adipose tissue browning mechanism.Methods: 32 SPF male SD rats at 3 weeks of age were randomly divided into 4groups: the standard diet quiet group(CS,n=8),the standard diet and exercise group(CE,n=8),the high-fat diet quiet group(HS,n=8),the high-fat diet and exercise group(HE,n=8).The quiet group didn’t exercise,the exercise groups were subjected to an 8-week aerobic exercise intervention program,5 days/week,total 60 minutes,and the slope of running platform was 10°.The exercise intervention program included 5 min warming up,50 min training,and 5 min rest.The standard diet group was fed with standard feed,and the high-fat diet group was fed with high-fat feed.After 8 weeks of intervention,all rats were measured for body weight and length.After taking blood from the abdominal aorta,the subcutaneous white fat and epididymis white fat were quickly separated and cut.Immunofluorescence laser confocal imaging was used to take tissue sections and observe the expression of PPARγ,PRDM16 and UCP-1.Western Blot was used to detect the expression of PGC-1α,PPARγ,PRDM16 and UCP-1 in adipose.Result:(1)Compared with the CS group,the body weight and adipose tissue weight of the rats in the CE group and the HE group were significantly reduced(P<0.05),and no significant difference in the Lee’s index of the rats.(2)In subcutaneous white adipose tissue: compared with the CS group,the expression levels of PPARγ and PRDM16 in the HS group were significantly increased(P <0.01),UCP-1 expression was significantly decreased(P <0.05),and there was no interaction between PPARγand PRDM16 in the tissue,UCP-1 fluorescence intensity increased(P <0.01);the expression of PGC-1α,PRDM16,and UCP-1 in the CE group was significantly reduce(P <0.05),the expression of PPARγ did not change significantly,the interaction between PPARγ and PRDM16 in the tissue increased slightly but not significantly,and the UCP-1 fluorescence intensity increased significantly(P < 0.01);compared with the HS group,the expression levels of PGC-1α,PPARγ,PRDM16,and UCP-1 in the HE group were significantly reduced(P <0.01),and the interaction between PPARγ and PRDM16 and UCP-1 fluorescence intensity were not significantly changed.(3)In epididymis white fat: compared with CS group,the expressions of PPARγ,PRDM16 and UCP-1 in HS group were significantly reduced(P <0.01),the expression of PGC-1α was significantly increased(P <0.05),and PPARγ and PRDM16 in tissue Interaction and UCP-1 fluorescence intensity have no difference;in CE group,PPAR [gamma] and UCP-1 group was significantly increased(P <0.05),PGC-1α expression was significantly decreased(P <0.01),PPARγ and PRDM16 interactions and UCP-1fluorescence intensity increased significantly(P <0.05);Compared with the HS group,the expressions of PGC-1α,PPARγ and UCP-1 in the HE group were significantly reduced(P <0.05),the expression of PRDM16 was significantly increased(P <0.01),and the interaction between PPARγ and PRDM16 was significantly increased(P <0.01),but there was no significant difference in UCP-1 fluorescence intensity.Conclusion: 1.The high-fat diet inhibits browning of white adipose tissue in rats,and the specific mechanism that influences the expression of PGC-1α,PPARγ,PRDM16,and UCP-1 needs to be further explored.2.Exercise can regulate white adipose browning through the interaction of PGC-1α,PPARγ and PRDM16 in white adipose tissue.In subcutaneous white adipose tissue,PGC-1α,PPARγ and PRDM16 are more likely to work together to transcriptionally regulate browning process.In the white fat tissue of the testis,it is possible to regulate the browning of white adipose by affecting the interaction of PPARγ and PRDM16,and exercise can improve the inhibitory effect of high fat diet. |
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