Correlation Analysis Of Low FFAR2 Gene Expression In Peripheral Blood And Acute Myocardial Infarction

Background and ObjectiveAcute myocardial infarction（AMI）is an acute disease with high mortality,which seriously threatens human health.More than 80% of cardiovascular disease deaths in the world are caused by AMI.Cardiovascular disease including AMI has become a major public health problem around the world.With the rapid development of economy and the change of lifestyle,the number of patients with AMI has increased sharply in China,and has become the main cause of hospitalization and death.As a kind of polygenic disease,the occurrence of AMI involves the interaction of genetic factors and environmental factors.However,some patients lack traditional risk factors related to the disease,which may promote AMI by being independent of traditional risk factors through susceptibility genes.Leukocytes in peripheral blood can be used as the evaluation window of cardiovascular diseases.Therefore,in order to explore new molecular marker for the diagnosis of AMI,more samples are required according to the previous gene chip results to verify the relationship between the expression of free fatty acid receptor 2（FFAR2）gene and AMI.Methods113 AMI patients hospitalized in the cardiology department of China-Japan Union Hospital of Jilin University from March 2018 to May 2018 were selected as the case group and 94 patients with non coronary heart disease as the control group.The relevant clinical data of the two groups were statistically analyzed,and the peripheral venous blood leukocytes of all patients were collected.Real-time polymerase chain reaction（RT-PCR）was used to detect the relative expression of the FFAR2 gene,and western blot analysis was used to detect the relative expression of the FFAR2 gene at the level of protein.The relationship between gene expression and clinical data was analyzed and compared.ResultsCompared with the control group（0.62[0.07,1.86]）,the m RNA relative expression level of FFAR2 gene in peripheral blood of AMI patients was lower（0.33 [0.04,1.08]）.The relative expression was 0.53 times that of the control group.The results of western blot showed that FFAR2 protein level was lower in AMI patients,which was 0.114 times that of the control group.The clinical data of the subjects showed that the patients of the AMI group were significantly older than that of the control group（P <0.01）,more people sufferd from type 2 diabetes（P =0.02）,the fasting blood glucose level was higher（P <0.01）,the high-density lipoprotein cholesterol（HDL-C）level was lower（P =0.03）,and the proportion of patients using hypoglycemic drugs was higher（P =0.01）.However,the patients in the two groups had no significant difference in history of hypertension,smoking history,serum triglyceride（TG）level,total cholesterol（TC）level and low density lipoprotein cholesterol（LDL-C）level（P >0.05）.The results of experimental analysis showed that the m RNA expression level of FFAR2 gene was correlated with HDL-C level（P<0.001）,but the m RNA expression level of FFAR2 gene was not correlated with age（P= 0.121）,type 2 diabetes（P=0.836）,and fasting plasma glucose level（P=0.339）.Logistic regression analysis showed that the low expression of FFAR2 gene in human peripheral blood was a risk factor independent of age,type2 diabetes,fasting blood glucose level and HDL-C level（P=0.025）.The low expression of FFAR2 increased the risk of AMI to 6.308 times.In addition,the high fasting blood glucose was also an independent risk factor leading to the disease（P=0.008）,and the high fasting blood glucose increased the risk of AMI to 3.132 times.ConclusionCompared with the control group,the expression level of FFAR2 gene in peripheral blood of patients with AMI was lower.The low expression of FFAR2 gene was an independent risk factor affecting the occurrence of AMI.Reduced expression of the FFAR2 gene in peripheral blood may be a potential biomarker for predicting risk of AMI.