Experimental Study Of Warming Acupuncture And Moxibustion At "Strong Acupoints" On Hashimoto’s Thyroiditis Rats

Objective:By observing the effect of warm acupuncture at Jianqiang point on serum thyroid protein antibody（TGAb）,anti thyroid peroxidase autoantibody（TPO AB）,thyroid microsomal antibody（TMAb）,interleukin-6（IL-6）,interleukin-12（IL-12）,free triiodothyronine（FT3）,free thyroxine（FT4）and to explore the effect and mechanism of warm acupuncture and moxibustion at Jianqiang point on Hashimoto’s thyroiditis（HT）in rats.Materials and Methods:Referring to the modeling method of Yang Yi^[1]:the modeling method of this experiment is as follows a total of 50 SPF female SD rats were used,aged6 weeks and weighing（110±10）g.The specific methods were as follows:In week 1,the rats were given adaptive feeding without intervention.At week 2,the rats were randomly divided into normal group、model group、acupuncture group、moxibustion group and warm acupuncture group,with 10 rats in each group.The normal group was given distilled water,and the other 4 groups were given high iodine water with a concentration of 0.64g/L.At week 3,the normal group was given distilled water,and the other 4 groups were still given high iodine water,while the rest remained unchanged.At week 4,rats in model group,acupuncture group,moxibustion group and warm acupuncture group were given subcutaneous multi-point injection of 0.2m L Tg（Tg:CFA=1:1）emulsified into oil-in-water by Fredrin’s complete adjuvant（CFA=1:1）in both feet,twice with an interval of 2 days,as the primary immunization,and no treatment was done in normal group.At week 5,rats in model group,acupuncture group,moxibustion group and warm acupuncture group were given subcutaneous multi-point injection of 0.2m L Tg（Tg:IFA=1:1）emulsified into oil-in-water emulsifier in both feet for 4 times,with an interval of 7 days,as enhancement of immunity,and no treatment was done in normal group.At the end of week 8,the modeling was complete.After modeling,venous blood was collected from the rat balls,and serum TPO-Ab level was determined by ELISA method.The success of modeling was judged by serum TPO-Ab level.The treatment started from the 9th week.In the acupuncture group,Dazhui,Shenshu（bilateral side）,Qihai,Guanyuan,Zusanli（bilateral side）points were acupuncture to obtain Qi and the needles were r EATined for15 minutes;in the moxibustion group,Dazhui,Shenshu（bilateral side）,Qihai,Guanyuan,Zusanli（bilateral side）acupoints were treated with mild moxibustion for 15 minutes;the warm acupuncture group took Dazhui,Shenshu（bilateral side）,Qihai,Guanyuan,Zusanli（bilateral side）acupuncture points.Keep the needle after the gas and insert a moxa column on the needle handle at the same time and light it for 15 minutes.After the 14th week of treatment,anesthesia was performed,and abdominal aortic blood was centrifuged,and the upper serum was taken to detect TGAb,TPO-Ab,TMAb,IL-6,IL-12 in rat serum by enzyme-linked immunosorbent assay（ELISA）.Serum FT3,FT4,TSH levels were determined by photochemical method,and the results were observed under microscope after HE staining of rat thyroid tissue.Results:1.Compared with the normal group,the levels of serum FT3 and FT4 in model group were significantly lower than that in the normal group（P<0.01）;The value of acupuncture group,moxibustion group and warm acupuncture group was significantly higher than that of the model group（P<0.01）.2.The levels of serum TSH,TGAb,TPO-Ab,TMAb,IL-6 and IL-12 in model group were significantly higher than those in normal group（P<0.01）;The values of acupuncture group,moxibustion group and warm acupuncture group were significantly lower than those of model group（P<0.01）.3.In terms of microscopic morphology of thyroid tissue,thyroid follicles in the normal group were oval,glandular epithelial cells were blank in morphology and structure,thyroid body secretion was normal without obvious inflammation.Compared with the normal group,the model group had abnormal thyroid structure and morphology,significantly increased the content of matrix in the follicle,accompanied by thyroid follicle atrophy,dotted infiltration of mast cells in the stroma,and hyperplasia of glandular epithelial cells.Compared with the model group,acupuncture group,moxibustion group and warm acupuncture group significantly reduced the content of matrix in thyroid follicles,only a small amount of follicle atrophy and inflammatory cell infiltration,and reduced the proliferation of glandular epithelial cells,but the morphological structure of warm acupuncture group was closer to that of the normal group.Conclusion:1.Acupuncture,moxibustion and warm moxibustion all increased the levels of serum FT3 and FT4 in HT rats,among which warm moxibustion had the most obvious effect,and the effects of acupuncture and moxibustion were similar.2.Acupuncture,moxibustion and warm moxibustion can decrease serum TSH,TGAb,TPO-Ab,TMAb,IL-6 and IL-12 in HT rats,among which warm moxibustion has the most obvious effect,and acupuncture and moxibustion have the same effect.3.Acupuncture,moxibustion and warm moxibustion can repair the thyroid tissue structure of HT rats to a certain extent,and reduce the inflammatory cells.Warm moxibustion has the most obvious effect,and the effects of acupuncture and moxibustion are similar.