| Objective Traditional pathological biopsy of lymph node resection has great trauma and time-consuming results,and it is difficult to excise and biopsy some special parts,which brings difficulties to diagnosis.In this study,a puncture specimen was selected by ultrasound-guided hollow-core needle biopsy technology to complete the detection of cellular immunophenotype by flow cytometry.At the same time,all the results were compared with the pathological diagnosis results of lymph node puncture biopsy,so as to establish a set of minimally invasive and rapid diagnosis process for patients with lymphadenopathy,thus winning the rescue time for critically ill patients.Methods 1.Patients admitted to the hospital from January 1,2020 to December 31,2021 who met the inclusion criteria were selected for lymph node ultrasound-guided hollow-core needle puncture.4-5 needles were taken,and the length of tissue cut by each needle was no less than 1cm.The sensitivity and specificity of flow cytometry in the diagnosis of lymphadenopathy were analyzed by comparing the results obtained by flow cytometry with pathological results.2.To evaluate the value of ultrasound-guided hollow-core needle biopsy combined with flow cytometry in the diagnosis of lymphadenopathy.Relevant data were analyzed by SPSS 25.0 statistical software.Results 1.A total of 89 patients were included in this study,including 77 cases of hollow-core needle puncture of lymph nodes guided by ultrasound,12 cases of lymph node resection,and 2 cases of follow-up lymph node resection.2.Among the 77 patients with lymphadenopathy,4 cases were pathologically diagnosed as metastatic carcinoma,6 cases were t-cell lymphoma,39 cases were B-cell lymphoma,1 case was small-cell carcinoma,and 27 cases were benign hyperplasia and descriptive report.Flow cytometry was used to detect 46 abnormal cells,including 4 cases of metastatic carcinoma,4 cases of T-cell lymphoma and 39 cases of B-cell lymphoma.3.Among the 12 patients with lymphadenopathy to be examined,1 case was pathologically diagnosed as metastatic carcinoma,2 cases as T-cell lymphoma,4 cases as B-cell lymphoma,and 5 cases as benign hyperplasia and descriptive report.Flow cytometry was used to detect abnormal cells in 7 cases,including 1 case of metastatic carcinoma,2 cases of T-cell lymphoma and 4 cases of B-cell lymphoma.The pathological diagnosis of lymph node resection was B-cell lymphoma in both 2 patients,and both hollow-core needle aspiration flow cytometry and pathological diagnosis were B-cell lymphoma.4.The diagnostic coincidence rate between flow cytometry and pathological diagnosis of B-cell lymphoma was 84.61%(33/39).The diagnostic coincidence rate of T-cell lymphoma was 66.67%(4/6).The diagnosis coincidence rate of metastatic carcinoma was 75.0%(3/4).5.The diagnostic coincidence rate of flow cytometry and pathological diagnosis for B-cell lymphoma was 100%(4/4);The diagnostic coincidence rate of T-cell lymphoma was 50.0%(1/2).The diagnosis coincidence rate of metastatic carcinoma was 100.0%(1/1).Conclusions Hollow-core needle aspiration combined with flow cytometry(10-color scheme)has certain application value in the diagnosis of patients with lymphadenopathy,especially for lymphoma.It plays an increasingly important role in the detection of mature lymphocytic tumors and follow-up after treatment,and has become an important auxiliary tool for pathological diagnosis.It can be used as a rapid method for the diagnosis of non-Hodgkin’s lymphoma and is worthy of further clinical application. |
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