Correlation Study On The Effect Of Sema3F On CREBBP Gene And Transcription Level In Primary Rat Hippocampal Neurons

Objective To culture primary rat hippocampal neurons in vitro to study the effect of Sema3 F on the expression and transcription level of c AMP-response Element Protein-binding Protein(CREBBP(CBP))gene in primary rat hippocampal neurons Sex,to provide a theoretical basis for the study of Sema3F-related diseases.Method 1.Take SD rats within 24 hours of newborn for in vitro culture of primary rat hippocampal neurons,divide the hippocampal neurons cultured to 72 hours into experimental group and control group;2.Experimental group: add the final concentration of 10ng/ml Sema3 F,control group: add the same concentration of fetal bovine serum,and the experimental group and control group will take samples of 0 minutes and 30 minutes respectively.3.Use high-throughput sequencing technology to perform m RNA sequencing on each sample.Use edge R software to quantify genes and calculate the Q value of gene expression between groups,and use these parameters to statistically screen differential genes.The SPSS22.0 statistical software was used to perform statistical analysis on the gene expression and transcript expression of the selected differential genes.The measurement data is analyzed by independent sample t test,and the obtained data were expressed by P<0.05 indicates that the difference is statistically significant.Results The expression of CREBBP DNA and RNA was up-regulated after adding Sema3 F for 30 minutes,and the difference was statistically significant compared with the control group.Conclusion 1.Sema3 F in primary rat hippocampal neurons up-regulated the expression of CREBBPDNA;2.Sema3 F in primary rat hippocampal neurons up-regulated the expression of CREBBPRNA.