Effect Of Sema3F On VEGF MRAN And Protein Expression In Primary Rat Hippocampal Neurons

Objective To investigate the correlation between the expression levels of Sema3 F and VEGF in primary rat hippocampal neurons,we used the mechanism of Sema3F-induced growth cone collapse in hippocampal neurons,and cultured the primary rat hippocampal neurons in vitro,which will provide theoretical basis and methods for treating diseases related to Sema3 F.Method 1.The Wistar rats were selected for new born in 24 hours,and the bilateral hippocampus was isolated and cultured.2.Primary rat hippocampal neurons cultured in the medium adding Sema3 F were collected after treated for 0,5,15,and 30 minutes.Primary rat hippocampal neurons cultured in the medium adding the same concentration of fetal bovine serum with Sema3 F were taken as control.3.Realtime-PCR and Western Blot techniques were used to detect the VEGF mRNA and its protein expression in all samples,respectively.The data were analyzed by repeated ANOVA using SPSS22.0 statistical software.For the statistical results,P<0.05 was considered as statistically significant.Results The expression of VEGF mRNA and protein was decreased at the transcriptional and translational levels after Sema3 F treated in the hippocampal neurons,but there was no significant change in the control.Conclusions 1.Primary rat hippocampal neurons were successfully cultured in vitro.2.Sema3 F can inhibit the expression of VEGF mRNA at transcriptional level in primary rat hippocampal neurons,and it is time-dependent.3.Sema3 F can induce a decrease in VEGF protein in primary rat hippocampal neurons,and it is time-dependent.