Expression And Clinical Significance Of Serum NLRP3 Inflammatory Bodies And AOPPs In Different TOAST Types Of Acute Cerebral Infarction

Objective: At present,many studies have proved that NLRP3 inflammatory bodies,and the late oxidative protein product(AOPPs),a new oxidative stress marker,participate in the process of atherosclerosis and the injury after cerebral ischemia.The role of NLRP3 inflammatory bodies and AOPPs on atherosclerosis is still in the research stage,and there are few studies on cerebral infarction and its subtypes,especially AOPPs and cerebral infarction.In this study,we detected the expression of patients ’ NLRP3 inflammatory bodies and AOPPs between different TOAST types of stroke,by means of exploring the expression level and clinical significance of NLRP3 inflammatory bodies and AOPPs in different TOAST types of cerebral ischemia,on main aim to provide some reference for the TOAST classification of acute stroke in clinical classification,and provide new ideas for the treatment and prevention of different subtypes of cerebral infarction in the futurelaboratory support for the determination of stroke ’ s TOAST classification and assist in the prevention and treatment of cerebral infarction.Methods: 1.We chose 57 patients with acute stroke as the experimental group,who admitted to the first affiliated Hospital of Hebei North University between August 2020 and December 2020.Depending on the TOAST classification of stroke,all cases of experimental group was segmented into three groups,including 33 case of large artery atherosclerotic stroke group(LAA group),14 case of small artery occlusive stroke group(SAO group),and 10 case of cardiogenic embolic stroke group(CE group).2.In the same period,chose 20 healthy cases in the same hospital for a control group.3.We collected the serum of 4 groups participants,then put the serum at indoor temperature to subside and centrifuge.At last,put the serum in a refrigerator at-80 ℃.4.The expression levels of NLRP3 inflammasomes and AOPPs were determined by ELISA.5.To compare the expression level of serum NLRP3 inflammatory bodies and AOPPs among 4 types,and acquire clinical significance.Results: 1.On basis of TOAST classification,77 participants were segmented into 4 groups,respectively is: LAA group(n = 33),SAO group(n = 14),CE group(n = 10)and control group(n = 20).After analysis test,between groups in gender,age,drinking,smoking,diabetes,hypertension factors,there was no statistically significant(p > 0.05).(See Table 1).2.The expression level of NLRP3 inflammatory bodies was higher in LAA group,SAO group,CE group than that in control group(see Fig1).The expression of NLRP3 inflammatory bodies between the four groups was statistically significant(F= 27.799,P < 0.001).(See Table2).3.The expression level of AOPPs in LAA group,SAO group,CE group was higher than that in control group(see Fig2).AOPPs expression between the four groups was significant difference(F=25.022,p < 0.001).(See Table3).4.The expression level of NLRP3 inflammatory bodies in SAO group was higher than that in LAA group and CE group,in LAA group was higher than that in CE group,with statistically significant(F=7.809,p=0.001).(See Table 4).5.The expression level of AOPPs in LAA group was higher than that in SAO group and CE group,in SAO group was higher than that in CE group.The difference was statistically significant(F=13.562,P < 0.001).(See Table 4).6.Pearsom related analysis showed that the expression of NLRP3 inflammatory bodies and AOPPs have significant positive correlation(r = 0.422,p < 0.001).(See Fig 3).7.Logistic regression analysis showed that AOPPs was a risk factor for major atherosclerotic cerebral infarction(OR=0.995,P =0.001).No statistically significant risk factors were found for other subtypes.(See Table 5).Conclusion:1.The expression levels of NLRP3 inflammatory bodies and AOPPs were increased in stroke group compared with the healthy control group.2.Serum NLRP3 inflammatory bodies and AOPPs were expressed differently in cerebral infarction subtypes.The expression level of NLRP3 was highest in SAO type,and that of AOPPs in LAA type was highest.This may have reference value for the early etiological classification of stroke.3.AOPPs was the risk factor of LAA type of stroke,which suggesting that AOPPs may is the biological marker for LAA subtype of stroke.4.Serum NLRP3 inflammasomes were positively correlated with the expression level of AOPPs.