| Long non-coding RNAs(lncRNAs)play an important role in tumor progression,In this study,the role of lncRNA ABHD11-AS1 in the progression of PTC was analyzed by detecting the expression of lncRNA ABHD11-AS1 in tissues of patients with Papillary thyroid carcinoma(PTC),as well as in vitro functional test and mechanism test.The mechanism of its action in PTC was further discussed.In this study,we collected 98 cases of PTC cancer tissues and their paracancerous tissues,and used paracancerous tissues as the control group to detect the expression of lncRNA ABHD11-AS1 in PTC tissues by qRTPCR,and the results showed that the expression of lncRNA ABHD11-AS1 in PTC tissues was significantly higher than that in paracancerous tissues.Then we used the chi-square test to analyze the influence of lncRNA ABHD11-AS1 on the prognosis of PTC patients,and the results showed that the high expression of lncRNA ABHD11-AS1 was related to lymph node metastasis in PTC patients.In order to further explore the role of lncRNA ABHD11-AS1 in the occurrence and development of PTC,two PTC cell lines,TPC-1 and KTC-1,were selected to transfection pc RNA3.1-ABHD11-AS1 or si-ABHD11-AS1,and p CDNA3.1 or si-NC was used as the control group.Transwell,MTT and other in vitro functional experiments were conducted,the results showed that inhibiting the expression of lncRNA ABHD11-AS1 could reduce the invasion,migration and proliferation ability of PTC cells.Consistent with the above results,overexpression of lncRNA ABHD11-AS1 significantly promoted the invasion,migration and proliferation of PTC cells.In this study,in order to explore the molecular mechanism of lncRNA ABHD11-AS1 in PTC,we found the downstream miR-29 a of lncRNA ABHD11-AS1 through RNA pull down and sequencing analysis,and predicted and screened the downstream target gene EPS15L1 of miR-29 a through two online bioinformatics databases(targetscan and Miranda).First,we performed qRT-PCR experiments on 98 cases of PTC patient tissues and their paracancerous tissues to detect the expression of miR-29 a in PTC.Using the paracancerous tissues as a control group,we found that miR-29 a was highly expressed in PTC.By transfecting TPC-1 and KTC-1 with miR-29 a mimics or miR-29 a inhibitor,using mimics-NC or inhibitor-NC as the control group,in vitro functional experiments such as transwell and MTT were performed,and the results proved miR-29 a overexpression could significantly promote the proliferation,invasion and migration of PTC cells,while inhibiting the expression of miR-29 a could significantly inhibit the proliferation,invasion and migration of PTC cells.And through the qRTPCR experiment,it was found that the expression of miR-29 a was significantly up-regulated after overexpression of lncRNA ABHD11-AS1,and the expression of miR-29 a was significantly down-regulated after the expression of lncRNA ABHD11-AS1 was inhibited.In other words,lncRNA ABHD11-AS1 may promote the progression of PTC through the positive regulation of miR-29a.Finally,in order to explore whether EPS15L1 was regulated by miR-29 a and lncRNA ABHD11-AS1,we applied qRT-PCR and western blot experiments to verify.The results showed that overexpression of miR-29 a could significantly promote the expression of EPS15L1 m RNA and protein levels.After inhibiting the expression of miR-29 a,the expression of EPS15L1 mRNA and protein levels was also significantly down-regulated.Consistent with the above results,after overexpression of lncRNA ABHD11-AS1,the expression of EPS15L1 m RNA and protein levels were also up-regulated,inhibiting the expression of lncRNA ABHD11-AS1,and EPS15L1 m RNA and protein levels were significantly decreased.In summary,the experimental results suggested that EPS15L1 was the downstream target of miR-29 a in PTC,and the expression of lncRNA ABHD11-AS1 in PTC was positively correlated with miR-29 a.Therefore,lncRNA ABHD11-AS1 promotes the progress of PTC through the miR-29a/EPS15L1 axis,which provides a new perspective for PTC diagnosis and treatment strategies. |
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