Construction Of Data Acquisition System Of Human-like Retinal Vision Chip

About 83%of the information in the human brain is acquired through the eyes,and the retina is the most important sensory structure in the eyes.The retina is a highly specialized neural tissue responsible for conversion of visible light into the electrochemical signal interpreted by the brain as vision,once retinopathy occurs,it may cause visual impairment or even loss of vision.Therefore,it is of great significance to study the structure of the retina and the generation and transmission of electrical signals.The microfluidic chip technology that has emerged in recent years provides a new idea for the construction of retinal chips.In this paper,a human retinal vision chip is fabricated based on laser direct writing technology,and conductive glass electrodes and gold electrodes are integrated into the chip to realize the detection of the potential of photoreceptor cells in the retina.The specific research contents are as follows:Firstly,according to the retina structure,the human retinal vision chip is designed by using Auto CAD2020 and other software,combined with the desktop maskless laser direct writing lithography machine,the produced mold is continuously improved and optimized,to solve the problem that the chip is easy to generate bubbles and the bubbles are difficult to discharge,difficult processing technology,photoresist displacement,deformation,adhesion,stretching,channel breakage and photoresist shedding,etc.The human retinal vision chip is bonded with the designed glass electrode or gold electrode by the plasma cleaner to make the final human retinal vision chip.Secondly,COMSOL Multiphysics software is used to simulate the hydrodynamics,particle tracking and carbon dioxide diffusion of part of the three-dimensional model of human retinal vision chip.Through the simulation of the three aspects of the human retinal vision chip,we have a preliminary understanding of the flow rate and pressure of the cell culture fluid flowing through the chip,the position of the cells,and the CO2 diffusion process of the chip in the cell incubator during the process of culturing cells.Finally,it is determined that the inoculation density with the fastest cell growth and the highest growth rate was 4×10~4-5×10~4 cells/m L,and the suitable passage time was 4-5 days.Inoculate retinal photoreceptor cells on the human retina vision chip to detect the potential,the results show that the changes of light and temperature will affect the potential of the cells,and the light will stimulate the cone and rod cells to produce potential changes.The change of temperature will affect the activity and state of the cell,thus indirectly affect the production of cell potential.The chip system can culture cells and detect the potential changes of cells stimulated by light and temperature.The process of the human retinal vision chip prepared in this study is simple and green,and the raw material is easy to obtain,and it can detect the potential changes produced by retinal photoreceptor cells.Compared with the existing retinal chips,the chip made in this study forms a multicellular network connection in the retina,which has certain advantages,is of great significance for the study of retinal structure and potential detection,and has potential biological and clinical application value in disease diagnosis.