Research On DNA Enrichment In Complex Sample Paper-based Microfluidic Chip Development And Control Technologies

Genes(fragments with genetic effects on DNA)are closely related to disease.Except for injuries and excessive hunger,almost all diseases can be detected by genetic risk,and early warning and targeted intervention can be carried out to target the disease.Take the initiative to improve your living environment and living habits,and prevent and avoid major diseases.The 2020 National Food Safety Risk Monitoring Plan,which was finalized by the National Food Safety Risk Assessment Center in 2019,emphasized the importance of conducting food-borne disease surveillance and effectively maintaining the health of people and diet safety.The detection of food-borne pathogens is mainly DNA detection.The technical problem currently encountered in the actual work of DNA detection is the high-sensitivity detection technology of target DNA(efficient DNA enrichment technology in complex samples).Therefore,this paper conducts research on DNA sample paper-based microfluidic chip enrichment and control technology in complex sample systems.The research is carried out in three levels.First,theoretical research is based on using COMSOL Multiphysics software.Second,DNA enrichment device is established under the guidance of theory Device,and then applied the device to quickly and efficiently enrich DNA.The main innovations in this article are as follows:1.A method of gravity-mediated field effect amplification and enrichment of charged analytes was proposed,and theoretical simulation based on COMSOL Multiphysics software was performed.The conductivity field,potential field,fluid field,and analyte concentration field were coupled for analysis and research.The effects of gravity mediation and electric field strength on enrichment results are shown.The simulation results are consistent with the experimental results,which verifies the feasibility of the theory.2.A simulation calculation strategy is proposed that analyzes the spatial and temporal scaling across orders of magnitude.The physical model of FASS is independently constructed,and a simplified dimensionless relationship between the concentration and width of FASS over time is obtained.The simulation results show that The micro-nano channel length and enrichment time were optimized to control the effects of molecular diffusion and convection diffusion,and a better analyte enrichment result was obtained.The enrichment ratio is positively correlated with the voltage value.It solves the non-uniform electricity in FASS by percolation,which causes the problem of unclear theoretical mechanism of analyte enrichment affected by molecular diffusion and convection diffusion.3.Independently designed and built a complex system DNA enrichment device,designed a smart and easy-to-use enrichment device that includes a power supply module,a light source module,an imaging module,a boost module,and a chip module in Solidworks software.A mobile phone app is written on the Android Studio platform.The device was used to achieve high-efficiency sample enrichment in a short time.The pigment was enriched 10 times in 60 seconds,and the two pigments were enriched and separated.Trace Norovirus pathological DNA was enriched 20 times in 100 seconds.