Research On The Microfluidic Technology For Detection And Sorting Of Cancerous Cells

Microfluidic technology has a wide range of applications in cell detection and sorting research.The scale size characteristics of the microfluidic chip just match the cell size,so the microfluidic chip can be used to operate on single cells or even multiple cells.This paper describes the research status and significance of cancer cell detection and sorting based on microfluidic technology,and proposes a research scheme that combines the principle of microstructural filtration with the principle of magnetic separation.The microfluidic technology for the detection and sorting of cancerous cells was studied from the aspects of theoretical analysis,numerical simulation,chip preparation and chip functional applications.In this paper,magnetic microspheres are used as models to analyze the magnetic microspheres in the microfluidic chip in detail,and the force equations of magnetic microspheres are deduced.Using COMSOL finite element software to solve the force equations of magnetic microspheres,and the magnetic microspheres in the microfluidic chip movement trajectory were obtained.The influence of the residual magnetic flux density of the permanent magnet and the inlet flow velocity of the microfluidic chip on the trajectory of the magnetic microsphere was analyzed.This paper describes the materials needed for chip preparation and the preparation process of the chip.A microfluidic experimental system was built based on the equipment required for the experiment,and the implementation of the magnetic nanoparticles carrying liver cancer cells(SMCC-7721)in the microfluidic chip Sorting,sorting rate is about 68.3%.From the experimental results,it can be seen that the magnitude and direction of the external force of SMCC-7721 cells carrying magnetic nanoparticles influence their trajectory in the microfluidic chip.