| Every outbreak of influenza virus is serious,it will cause high infection rate and high death rate,and cause devastating blow to people’s life and social economy.There are many influenza virus subtypes.As an RNA segmented virus,the gene recombination rate between different strains is very high,and it has a very high mutation rate.It is very difficult to accurately detect the virus type.The main basis for influenza virus typing is the two surface glycoproteins of the virus,namely hemagglutinin(HA)and neuraminidase(NA).Based on the difference between these two antigens,influenza A virus has the most subtypes and is the most harmful to humans.Its subtypes can be divided into 16 H subtypes and 9 N subtypes.The research object of this paper is mainly for the detection and sorting of HA of influenza A virus subtypes.In this project,an automatic sampling platform is designed for the sampling of multiple reagents,and two microfluidic chips are designed for the HA protein of the virus.,Studying different virus detection methods provides two feasible detection methods for the early diagnosis of influenza virus.At the same time,the highly integrated and automated operation of the detection platform can provide a very good guarantee for the safety of detection personnel.A valve-controlled microfluidic chip is designed in the automatic sampling device to control the circulation of multiple reagents.Through the host computer control program written by Labview,the closing of the microvalve in the valve-controlled chip and the liquid flow rate are controlled to achieve Automatic sampling of multiple reagents.For the two detection chip designs for virus HA,first,in the first detection method,multiple physical fields are combined to develop a magnetic and size-mediated microfluidic chip,using magnetic microspheres of different sizes as A variety of influenza virus capture carriers are used for magnetic separation and size sorting.In the detection experiment,H7N9 HA and H9N2 HA are used as the detection objects for simultaneous detection,and quantum dots are used as detection probes,and the fluorescence intensity of the quantum dots is indirect.Determination of virus type and concentration,the experimental results showed good detection performance and high sensitivity,as well as excellent specificity and good reproducibility,indicating that this detection method can be applied to the detection of a variety of influenza viruses.Secondly,in the second detection method,a microfluidic chip based on the three-electrode system in electrochemistry was developed.Gold was used as the electrode material and the catalyst HRP(horseradish peroxidase)was used as the detection probe.Promote the formation of a reversible redox reaction between HQ(hydroquinone)and BQ(p-benzoquinone).When the converted BQ is converted to HQ again under the action of the electrode,a reduction current will be generated,using differential pulse voltammetry(DPV))Detect the current change,and test the H7N9 HA virus through experiments.The final result shows that this detection method is also feasible.These two detection methods combine electronic technology with microfluidic chip technology to give full play to their respective advantages and provide new ideas and new ways for early and efficient detection of multiple influenza subtypes.In practical applications,it can reduce the detection personnel’s The difficulty of operation and the risk of infection also have great application potential in low-concentration virus detection. |
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