The Molecular Mechanism Of MiR-204-5p Regulation In Promoting The Migration And Invasion Of Bladder Cancer Cells

Background:Bladder cancer is one of the most common malignant tumors of the urinary system.The incidence of bladder cancer is increasing year by year.However,the molecular mechanism of the development and progression of bladder cancer in the organism has not been fully elucidated.Bioinformatics analysis revealed that miR-204-5p is closely related to the progression of bladder cancer,and studies have confirmed that miR-204-5p can regulate gene expression,providing a basis for elucidating the mechanism of the development and progression of bladder cancer.Objective:The role of miR-204-5p in bladder cancer was demonstrated by a variety of nuclear and molecular biology techniques,and the molecular mechanism of miR-204-5p promoting the migration and invasion of bladder cancer cells by regulating genes was clarified.Methods:From the TCGAwebsite(https://portal.gdc.cancer.gov/)to download(BLCA),437 cases of bladdercancer ofmicrornas rawdata(datatype: Isoform Expression Quantification,Workflowtype: BCGSC miRNA Profiling)and thecorresponding clinical pathological characteristics andsurvivalinformation.Normal samples and incomplete clinicalrecords were excluded.395 BLCApatients eventually beincorporated inthesubsequent analysis,through bioinformaticsanalysis found that miR-204-5 p is closely related to the progress of thebladder,build miR-204-5 p stable expression and thesilenceof the bladdercancer cell lines,observation of miR-204-5 p afterexpressing andsilencebladder cancercell migration and the changeof the invasiveability,confirmed that miR-204-5 p effects on bladder cancercell migration and invasion ability.Results:1.RX64 3.6.1 Software analyzed the data,and bioinformatics analysis showed that miR-204-5p was closelyrelated to theprogression of bladdercancer.2.Hsa-miR-204-5p was differentially expressed in BLCA,and the differentially expressed miR-204-5p was statistically different with different clinicopathological features,and the OS with high expression was worse(P < 0.05).3.Hsa-miR-204-5p showed statistical significance in COX univariate analysis and COX multivariate analysis in BLCA(P < 0.05).4.There were a total of 866 target genes of hsa-miR-204-5p in the three prediction databases(those with more than 2 predicted positive genes),and 866 target genes were enriched in GO and KEGG,which were found to be related to HIPPO,Wnt and other signaling pathways(P< 0.05).5.The scratch and Transwell experiments confirmed that the migration and invasion ability of bladder cancer cells were enhanced after the overexpression of miR-204-5p,but decreased afterthe silencing,withstatistical significance(P < 0.05).Conclusion:HSA-miR-204-5p is differentially expressed in BLCA,which is closely related to different clinicopathological characteristics.After overexpression of miR-204-5p,the migration and invasion ability of bladder cancer cells is enhanced,while after silencing of miR-204-5p,the migration and invasion ability of bladder cancer cells is weakened,suggesting that miR-204-5p affects themigration and invasion ability of bladdercancer cells through regulation.