| Objective:This experiment detected the expression of PI3K pathway related key proteins PI3K,AKT,and mTOR,which were divided into the following three groups:normal ovarian tissues,benign ovarian tumors,and epithelial ovarian cancer.This experiment detects the expression of PI3K pathway related key proteins PI3K,AKT and mTOR,which were divided into the following three groups:normal ovarian tissue(control group),ovarian benign tumor(benign group),and epithelial ovarian cancer(malignant group).Then analyze the expression and significance of those related proteins of this pathway in EOC with different clinicopathological characteristics,and explore whether there is a correlation among the expression of three proteins,so as to lay the foundation for molecular targeted therapy of ovarian cancer.Methods:Collected normal ovarian tissues(30 cases),benign ovarian tumors(30cases),epithelial ovarian cancer group(46 cases)from September 2015 to December 2020in the Affiliated Hospital of Yan’an University,and detected the expression of three related proteins PI3K,AKT and mTOR by immunohistochemistry in three groups.Furthermore,collected the age,BMI,target ovarian tumor markers(HE4,CEA,CA-125 and CA-724),histopathological type,FIGO staging,differentiation level,Whether it has lymph node metastasis,and whether it is accompanied by peritoneal implant metastasis of epithelial ovarian cancer(EOC)patients,combined with immunohistochemistry and clinicopathological data for statistical analysis,explore the expression of PI3K pathway related proteins PI3K,AKT and mTOR in EOC tissues and their clinical pathological characteristics and correlation of their expression in EOC patients.The results of the experiment were sorted out and analyzed by SPSS 25.0.Results:1.The average age distribution of the three groups is:55.15±8.99 years old in the malignant group,39.43±11.70 years old in the benign group,and 52.70±4.88 years old in the healthy control group.The average age of the malignant group is higher than that of the benign group,and the difference is statistically significant(P<0.05);When comparing the body mass index of the three groups,the average BMI of the malignant group was 22.90 Kg/m~2,the average BMI of the benign group was 23.07 Kg/m~2,and the average BMI of the normal group was 24.56 Kg/m~2.The BMI of the malignant group was significantly higher than that of the control group.The difference is statistically significant(P<0.05).Among 46 patients in the malignant group,1 person was under 40 years old,accounting for about 2.17%of the total number,11 persons aged 40 to 49 years old,accounting for approximately 23.91%of the total number,19 persons aged 50 to 59 years old,accounting for approximately 41.30%of the total number,13 persons aged 60 to 69years old,accounting for about 28.26%of the total number,and 2 persons aged over 70years old,accounting for about 4.35%of the total number.2.HE4,CA-125,CA-724 in the malignant group were significantly higher than those in the benign group,the difference was statistically significant(P<0.05),but there was no significant difference in CEA between the two groups(P>0.05).3.The expression of PI3K protein in normal ovarian tissues,benign ovarian tumor tissues,and epithelial ovarian cancer showed a gradually increasing trend,and the difference was statistically significant(P<0.05);Among them,the positive rate of expression in the malignant group was higher than benign group and healthy control group,the difference was statistically significant(P<0.0167).4.The expression of AKT protein in normal ovarian tissue,ovarian benign tumor tissue,and epithelial ovarian cancer showed a gradual increase trend,and the difference was statistically significant(P<0.05);Among them,the positive rate of expression in the malignant group was higher than benign group and healthy control group,the difference was statistically significant(P<0.0167).5.The expression of mTOR protein in normal ovarian tissue,ovarian benign tumor tissue,and epithelial ovarian cancer showed a gradually increasing trend,and the difference was statistically significant(P<0.05);Among them,the positive rate of expression in the malignant group was higher than benign group and healthy control group,the difference was statistically significant(P<0.0167).6.There was no significant difference between the expression of three proteins in the EOC group and the four ovarian tumor markers(P>0.05).7.The expression levels of PI3K and mTOR are related to the staging,lymph node metastasis and peritoneal metastasis of epithelial ovarian cancer(P<0.05),but are not related to the age,histological type and degree of differentiation of patients with ovarian cancer(P>0.05).The expression level of AKT is related to the staging of epithelial ovarian cancer(P<0.05),but has nothing to do with the age,histological type,degree of differentiation,lymph node metastasis and peritoneal metastasis of patients with ovarian cancer(P>0.05).8.In epithelial ovarian cancer tissues,the expression of PI3K and mTOR were positively correlated through spearman correlation analysis,with an r_s value of 0.631,P<0.05;The expression of AKT and mTOR were positively correlated with an r_s value of0.351,P<0.05;the expression of PI3K and AKT were positively correlated with an r_s value of 0.305,P<0.05.Conclusions:1.The expression of PI3K,AKT,and mTOR in normal ovarian tissue,benign ovarian tumor tissue,and epithelial ovarian cancer showed a gradual increasing trend,suggesting that the positive expression of the three proteins is related to the occurrence and development of ovarian cancer.2.The expression of PI3K protein and mTOR protein is related to the FIGO staging,the presence or absence of lymph node metastasis and peritoneal metastasis of EOC patients;the expression of AKT protein is related to the FIGO staging of EOC patients.3.The expressions of PI3K,AKT,and mTOR are positively correlated.The three proteins may be involved in the occurrence and development of ovarian cancer,suggesting that inhibiting this signaling pathway may be a new approach for clinical treatment of ovarian cancer. |
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