| Objective:To investigate the effect of potassium iodate on proliferation,migration and invasion of papillary thyroid carcinoma TPC-1 cells in vitro and its potential molecular mechanism.Methods:TPC-1 cells were cultured and passaged in vitro.The cells were treated with 0 mol/L,10-10mol/L,10-8mol/L,10-6mol/L,10-4mol/L and 10-2mol/L potassium iodate.MTT test,scratch test and Transwell test were used to calculate the relative proliferation rate,migration index and the number of penetrating cells,and the proliferation,migration and invasion ability of TPC-1 cells were analyzed.Then,the m RNA and protein expressions ofβ-catenin,c-myc and cyclin D1,the key downstream targets of Wnt/β-catenin signaling pathway in TPC-1 cells were detected by real-time fluorescent quantitative RT-PCR and Western blot,respectively.The molecular mechanism of potassium iodate on proliferation,migration and invasion of TPC-1 cells in vitro was analyzed.Results:1.Cell proliferation,migration and invasion:The relative proliferation rate,migration index and number of penetrating cells of TPC-1 cells were positively correlated with the concentration of potassium iodate in the range of(0-10-6)mol/L.The relative proliferation rate,migration index and number of penetrating cells reached the largest in the 10-6mol/L group,which were 1.17±0.03,（92.80±0.84）%and（107.80±2.86）,all of which were significantly higher than those in the control group（P<0.001）.The relative proliferation rate,migration index and number of penetrating cells were negatively correlated with the concentration of potassium iodate in the range of(10-6-10-2)mol/L.The relative proliferation rate,migration index and number of penetrating cells of TPC-1 cells in 10-2mol/L group were the lowest,followed by0.92±0.04,（60.20±1.48）%,（35.40±2.97）,respectively,which was significantly lower than that in control group（P<0.001）.2.m RNA expression level:q RT-PCR was used to detect the m RNA expression level ofβ-catenin,c-myc and cyclin D1 downstream genes of Wnt/β-catenin signaling pathway in TPC-1 cells at 48 h.When the concentrations of potassium iodate was in the range of(0-10-6)mol/L,the relative m RNA expression level ofβ-catenin,c-myc and cyclin D1 were positively correlated with the concentrations.The relative expression was the highest in 10-6mol/L group,followed by 1.57±0.07,1.25±0.06,1.44±0.03,respectively,which was significantly higher than that in control group（P<0.001）.When the concentration of potassium iodate was in the range of(10-6-10-2)mol/L,the m RNA expression levels ofβ-catenin,c-myc and cyclin D1 were negatively correlated with the concentrations of potassium iodate.The m RNA relative expression was the lowest in 10-2mol/L group,which was 0.94±0.04,0.84±0.05,0.96±0.03,respectively,which was significantly lower than that in control group（P<0.001）.3.Protein expression level:the relative protein expression ofβ-catenin,c-myc and cyclin D1 were detected by Western blot.The relative protein expression ofβ-catenin、c-myc and cyclin D1 was positively correlated with the concentration in the range of(0-10-6)mol/L potassium iodate.The relative protein expression of 10-6mol/L group was0.90±0.03,0.84±0.02,0.89±0.02,respectively,which was significantly higher than that of control group（P<0.001）.The relative protein expression of TPC-1 cellβ-catenin、c-myc and cyclin D1 in the range of(10-6-10-2)mol/L potassium iodate concentration was negatively correlated with potassium iodate concentration,and the relative protein expression of the three groups in 10-2mol/L group was0.63±0.03,0.50±0.03,0.51±0.01,respectively,which were significantly lower than those in the control group（P<0.05）.Conclusion:1.(0-10-6)mol/L potassium iodate can promote the proliferation,migration and invasion of papillary thyroid carcinoma cells by upregulating the expression ofβ-catenin、c-myc、cyclin D1 in the Wnt/β-catenin signaling pathway.2.(10-6-10-2)mol/L potassium iodate can inhibit the proliferation,migration and invasion ofpapillary thyroid carcinoma cells by down-regulatingβ-catenin、c-myc、cyclin D1 expression in the Wnt/β-catenin signaling pathway. |
PDF Full Text Request