Detection And Significance Of Treg、IL-6,IL-17 And TNF-α In The Peripheral Blood Of Patients With Alopecia Areata

Background and purposeAlopecia areata(AA)is a common-seen hair-loss disease in dermatology clinics,manifested as a sudden occurrence of non-scarring hair loss,which can be seen in any hair growth throughout the body.The hair loss spots are generally round or oval,Hairs at the edge of spots look like exclamation marks.Local skin behaves as normal,without any inflammatory responses.The prognosis of AA usually promises a good prognosis,most patients with mild AA can be self-healing,whereas some patients develop to alopecia totalis(AT)and alopecia universalis(AU),about whom mental health can be affected.At present,the cause and pathogenesis of AA is not clear.It is generally believed that T-cells mediate autoimmune disease,and hair follicles are its target organs.CD4+T cells and CD8+T cells were involved in the onset of AA.Depending on the secretion of cytokines,CD4+T cells can be divided to helper T cells 1(Th1),Th2,Th9,Th17,Th22 and regulatory T cells(Treg).It is found that initial T cells(Th0)can differentiated to Th17 with the help of IL-6,through which IL-6 can maintain the balance of Th17 and Treg.IL-17 is the main and most characteristic cytokines produced by Th17,which can promote autoimmune diseases through a variety of signaling paths.At present,it has been found that there is a large amount of peripheral blood and tissue in patients with various autoimmune diseases such as systemic lupus erythematosus,rheumatoid arthritis,asthma and multiple sclerosis.Treg is one of the classes of T lymphocyte subsets performing immunosuppression and immune tolerance.,including the inflammation,allergy,tumor immunity and other processes.Recent studies have found that Treg can promote hair growth by promoting hair follicle stem cell proliferation and diferentiation.Hair loss can be leaded by the reducing amount of Treg.Geneme-Wide Association Studies have shown that most of the 14 susceptibility gene site found so far is functionally related to Treg,speculating that Treg may be involved in the onset of AA and involves many of signal pathways.The purpose of this study is to explore the possible pathogenesis and to provide potential new method of treatment by analyzing the levels and changes of Treg,I L-6 and IL-17 in the peripheral blood of patients with AA.MethodChose 23 active AA patients as test group,which all came from the The First Affiliated Hospital of Zhengzhou University.They all have confirmed and complete case data.21 age-and sex-matched consenting healthy volunteers were enrolled as controls,which all came from the health examination center of the First Affiliated Hospital of Zhengzhou University.This group includes 11 males and 10 females.The mean ages of the patients with AA and the controls were 30.71 ±4.46 years and 33.00± 4.18 years.Collect 5ml peripheral venous blood simple from each AA patients and volunteers,and use flow cytometry method to detect the absolute concentration of Tregs,IL-6,IL-17 and TNF-α.Use SPSS 21.0 Software to analyzes data.The data will be described by mean±standard deviation or quartile.Results1.The concentration of IL-6 in the serum of patients with AA was 1.43(1.30±1.81)pg/ml,lower than that of the control group 1.50(1.32±1.91)pg/ml,and the difference was not statistically significant(Z=-0.537,P=0.591,P>0.05);The concentration of IL-17 in the serum of patients with AA was 4.28±4.05pg/ml,higher than the control group 3.63±4.74pg/ml.and this difference is not statistically significant(Z=0.489,P=0.745,P>0.05).he concentration of TNF-α in the patient’s serum is 1.28(0.98±0.54)pg/ml,lower than the control group IL-6 concentration of 1.22(1.03±1.62)pg/ml,the difference is not statistically significant(Z=-0.158,P=0.874,P>0.05).2.IL-17 concentrations in patients with mild illness are 3.59(0.59±5.55)pg/ml,lower than the severe group 5.94(0.29±835)pg/ml,and the difference is not statistically significant(Z=-0.806,P=0.420,P>0.05);The concentrations of IL-6 in patients with mild illness were 1.43(1.25±1.68)pg/ml,lower than the severe group 1.53(1.43±1.81)pg/ml,and the difference was not statistically significant(Z=-1.096,P=0.273,P>0.05);The concentration of TNF-α in patients with mild illness was 1.08(0.92±1.53)pg/ml,lower than that of severe group 1.53(1.27±1.89)pg/ml,and the difference was not statistically significant(Z=-1.783,P=-0.075,P>0.05).3.The absolute number of Treg cell in the peripheral blood of patients with AA was 50.46±34.98(/l),higher than the number of health control group about 36.92±23.42(/l),and the difference was statistically significant(t=9.468,P<0.01).Conclusion1.The absolute value of Treg cells in the peripheral blood of patients with alopecia areata increased significantly,indicating that Treg cells are involved in the pathogenesis of AA.2.The differences between IL-6,IL-17 and TNF-α in the mild group and severe patients was not statistically significant,indicating that the levels of these three cytokines were not related to the severity of AA.3.The differences between the serum levels of IL-6,IL-17 and TNF-α in the AA group and health control patients was not statistically significant suggesting the levels in the serum of IL-6,IL-17,TNF-α were not related to the pathogenesis of AA.