Extraction Of Anthraquinones From Seeds Of Senna Occidentalis And Its Antioxidant Activity

Senna occidentalis L.,a Latin synonym:Cassia occidentalis L.,which is a subshrub or shrub of the genus Cassia in the leguminous family.It was distributed in the southeast,south and southwestern provinces of China and the area south of the Yangtze River,was a common wild plant in riverside beach and a village wasteland.Its main component was anthraquinone,which was also the secondary metabolite with the highest content.This study established an methods of ultraviolet spectrophotometric system to determin anthraquinone from S.occidentalis seeds by screening different solvents to extract the quinone components from the seeds,Taking the extraction rate of anthraquinone components as an indicator,the optimal extraction conditions were obtained through single factor and response surface optimization,and the components of the extract were detected by LC-MS,and the in vitro antioxidant activity of the effective components in S.occidentalis seeds were determined.1.The best solvent for extracting anthraquinones from Senna occidentalis seeds was selected through Soxhlet extraction method.In the study,petroleum ether,ethyl acetate,chloroform,n-hexane,n-hexane+distilled water,distilled water,ethanol and other solvents were used to extract the bacteria.Awakening substances,the optimal extraction solvent is screened out.The results shows that ethanol is the best solvent for extracting the components of anthraquinone awakening from the Senna occidentalis L.seeds,with the advantages of low toxicity,easy availability and low cost.The UV spectrophotometer test system of anthraquinone components of Senna occidentalis L.seeds was established through the exploration of standard and samples.2.Exploring the single factor of ultrasonic extraction and its response surface optimization by selecting the best reagent,and using the Design-Expert software to design the extraction temperature,extraction time,and material-to-liquid ratio according to the optimal extraction range of the influence factor obtained in the single-factor experiment.The response surface optimization experiment of four factors and three levels of solvent concentration finally obtained the optimal extraction conditions of Senna occidentalis L.awake components,and carried out the optimal verification.Through experimental research,the results shows that the ethanol concentration is 70%,the material-to-liquid ratio is 1:20,the extraction temperature is 60℃,and the extraction time is 30min,the highest content of bacterial aldehydes.3.Explore the single factor of microwave extraction and its response surface optimization by selecting the best reagents,and use the Design-Expert software to design the extraction power,extraction time,and material-to-liquid ratio according to the optimal extraction range of the influence factor obtained in the single-factor experiment,The response surface optimization experiment of four factors and three levels of solvent concentration finally obtained the optimal extraction conditions for the components of Senna occidentalis L.,and performed the optimal verification.The results shows that the ethanol concentration is 70%,the material-liquid ratio was 1:20,the extraction power was 350W,and the extraction time was 14min.The content of anthraquinone was the highest.4.The extract obtained under ultrasonic and microwave organic solvent extraction uses LC-MS to detect the components of the extract,and analyze the other components of the bacterin,as well as the types and quantities of other chemical components.5.The anti-oxidation study was carried out by extracting the anthraquinone awakening substances under the optimal conditions of ultrasound and microwave,setting different concentration gradients,and carrying out the anti-oxidation study to analyze the clearance rate of the contained components.The study found that with the concentration gradient of the anthraquinone awakening substances extracted from Senna occidentalis L.seeds in the experiment,DPPH,TRAP,ABTS and other antioxidant methods.found that the higher the concentration,the better the antioxidant effect.