The Effect Of Protosappanin B On The Proliferation,apoptosis And Migration In Colon Cancer SW620 Cell

Objective:Colorectal cancer is one of the most common cancers in China.At present,the treatment of colorectal cancer mainly includes surgical resection,chemotherapy,radiotherapy and targeted drug therapy.However,the high cost of comprehensive treatment leads to heavy economic burden on patients with advanced colorectal cancer in many areas of China.Traditional Chinese medicine has a certain price advantage in the treatment of cancer,many literatures reported that some traditional Chinese medicine extracts had anticancer effect,Protosappanin B（PSB）is one of the main components of the Lignum Sappan extract of traditional Chinese medicine,and the anticancer effect may make it a potential new preparation against colon cancer.The purpose of this study was to investigate the effect of PSB on colon cancer cells in vitro.Methods:1.The effect of PSB on the survival of colon cancer SW620 cells:colon cancer SW620 cells were treated with different concentrations of PSB（0,6.25,12.5,25,50,100,200μg/m L）,the survival rate of colon cancer SW620 cells was measured by CCK-8 method,and the IC₅₀ of colon cancer SW620 cells inhibited by PSB was calculated.2.SW620 cell grouping:the colon cancer SW620 cells were divided into four groups:（1）Control group:human colon cancer SW620 cells;（2）Experimental group 1:colon cancer SW620 cells treated with 17.5μg/m L PSB;（3）Experimental group 2:colon cancer SW620 cells treated with 35μg/m L PSB;（4）experimental group 3:colon cancer SW620 cells treated with 70μg/m L PSB.3.The cell proliferation vitality of each group was detected by CCK-8 method and plate clone formation experiment,apoptosis rate of each group was analysed by Annexin V-FITC/PI flow cytometry,and cell migration ability was measured by Transwell method.4.The effect of PSB on colon cancer SW620 cells was analysed through the comparion of cell proliferation,apoptosis and migration in each group.Results:1.Detection of the survival rate in SW620 cells by CCK-8 method:with the increase of concentration and treatment time of PBS,the survival rate of SW620 cell gradually decreased,showing a concentration and time-dependent manner;the IC₅₀ of SW620 cell was 24h:IC₅₀=35.25μg/m L,48h:IC₅₀=24.66μg/m L,72h:IC₅₀=12.82μg/m L,96h:IC₅₀=9.49μg/m L,respectively.Therefore,the concentration of PSB was selected as 35μg/m L in the following intervention experiment.2.The estimation of proliferation vitality in SW620 cells by plate clone formation assay:the proliferation vitality of SW620 cells decreased with the increase of the concentration of PSB（17.5μg/m L、35μg/m L、70μg/m L）,there were significant difference between the control group and the experimental groups,but also between the experimental groups（P<0.001）.3.The analysis of apoptosis rate in SW620 cells by Annexin V-FITC/PI flow cytometry:the apoptosis rate of SW620 cells treated with IC50 PBS was significantly higher than that in control group,the difference is statistically significant（P<0.001）.4.The measurement of cell migration ability in SW620 cells by Transwell method:the migration number of SW620 cells treated with IC50 PBS was significantly lower than that in control group,the difference is statistically significant（P<0.001）.Conclusion:PSB can inhibit the proliferation and migration of colon cancer SW620 cells and promote its apoptosis.The anti-tumor effect suggests that PSB may be a potential new drug for colon cancer.