| BackgroundColon cancer is one of the most common clinical malignant tumors of the digestive tract,and has become one of the three most common tumors in the world.According to data published by the World Health Organization(WHO),colorectal cancer is the third most prone tumor in men,second only to lung cancer and stomach cancer;it is the second most prone tumor in women,second only to breast cancer,There are more than 1 million new cases of colorectal cancer worldwide each year.In my country,the mortality rate of colorectal cancer is second only to lung cancer,liver cancer,stomach cancer,and esophageal cancer,ranking fifth,seriously endangering the health of residents,and placing a heavy burden on society and the family economy.MF is an effective glucocorticoid.It is a high affinity synthetic 17-heterocyclic steroid steroid.It is used for local treatment of inflammation on the skin,lungs and nose.It has the function of suppressing immune response and mediator release and promoting mediator metabolism Has various effects,such as alleviating related symptoms,and has a wide range of pharmacological effects.Although there are a lot of research reports on colon cancer at home and abroad,there are few studies on the inhibitory effect of MF on colon cancer.ObjectiveStudy the effects of MF on the proliferation,apoptosis and migration of colon cancer cell lines in RKO and LoVo through cell biology experiments in vitro;in order to clarify the role and mechanism of MF in colon cancer cells and lay the theoretical foundation for understanding the colon cancer.Methods1.CCK8(Cell Counting Kit-8)experiment was used to detect the effect of MF on the proliferation ability of colon cancer cell lines RKO and LoVo.The MF concentration was set to 0μM,1μM,10μM,20μM,50μM,100μM,and the action time was 0,24 h,48h,and72 h.The optimal time and concentration were selected for plate clone formation experiments.2.To analyze the effect of MF on the apoptosis of colon cancer cells by flow cytometry.3.Scratch healing test and Transwell migration test were used to detect the effect of MF on the migration ability of colon cancer cell lines.4.Real-time quantitative polymerase chain reaction(qPCR)and Western blotting were used to detect the expression of proliferation,apoptosis,migration related mRNA and protein in colon cancer cell line after MF treatment.Results1.Cell CCK8 test results showed that mometasone furoate affected colon cancer cell lines RKO and LoVo.Compared with the control group,the growth rate of the mometasone furoate experimental group was significantly slower(P <0.05),and was time-dependent.And concentration-dependent,the optimal concentration(20μM)and the optimal time(48h)were selected for subsequent experiments to continue the verification;the results of the plate clone formation experiment showed that the number of plate clones in the experimental group of mometasone furoate was significantly less than the control Group cells(P <0.05).2.Flow cytometry experiment showed that the apoptosis rate of mometasone furoate experimental group was significantly higher than that of the control group(P <0.05).3.The results of cell scratch healing experiments showed that the cell migration speed of the experimental group after the action of mometasone furoate was significantly lower than that of the control group(P <0.05);The number of membranes was significantly less than that of the control group(P <0.05).4.Real-time quantitative polymerase chain reaction qRT-PCR experiments,the results showed that the proliferation-related mRNA,migration-related mRNA,and EMT-related mRNA of the mometasone furoate experimental group were significantly reduced compared with the control group(P <0.05).5.Western blotting experiments,the results showed that compared with the control group,the cells of the mometasone furoate experimental group were significantly reduced in proliferation-related proteins,migration-related proteins,and EMT-related proteins compared with the control group(P <0.05).Conclusions1.Mometasone furoate inhibits colon cancer cell proliferation and promotes apoptosis in vitro;2.Mometasone furoate inhibits the invasion and migration of colon cancer cells in vitro;3.Mometasone furoate may inhibit PI3 K / AKT / mTOR signaling pathway. |
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