Study On The Relationship Between Psoriasis Vulgaris And VDR Gene In Hainan Han Population Based On Target Gene Capture Sequencing

Objectives To investigate the relationship between VDR gene polymorphisms and psoriasis vulgaris.Methods A total of 79 patients with psoriasis vulgaris and 101 healthy controls were enrolled into this study,and they were all of Han nationality from Hainan province.The target gene capture sequencing method was used to sequence the full length of the VDR gene and the regions of 2kb up-and down-stream of this gene.Single nucleotide polymorphism(SNP)-based association analysis and haplotype-based association analysis were performed in SNPs with minimum allele frequency greater than 1%.Bioinformatics methods were also used to predict the impact of risk SNP on gene function.Results SNP-based association analyses showed that 40 SNPs(29 in introns,1 in exon,and 10 in intergenic regions)were susceptible to psoriasis vulgaris(OR=0.148～2.779,95%CI=0.016～6.130,P=0.000～0.976).Bioinformatic prediction indicates that rs2228570,located in exon,can lead to amino acid change,which in turn impacts the gene function.Haplotype-based association analyses showed that 10 haplotypes were the protective haplotypes of disease(OR=0.198～0.630,95%CI=0.039～0.985,P=0.002～0.048),and the frequencies of the haplotypes were from 5.150% to 45.570%in controls and 1.110% to 33.170% in patients,respectively.Conclusions VDR gene is associated with the pathogenesis of psoriasis vulgaris in Hainan Han population.