| In recent years,photothermal therapy has shown promising application in clinical treatment due to its high efficiency,low toxicity and non-immunogenicity in melanoma.Although various photothermal agents have been extensively studied,photothermal therapy still has many defects.For example,most of photothermal agents are difficult to degrade in the human body due to harmful effects,long-term cytotoxicity,and cannot promote wound healing at the treatment site which leads to its obstruction in clinical trials.Therefore,it is imperative to develop a photothermal scaffold materials that can achieve the treatment of melanoma and promote wound healing at the treatment site.The main contents of this study include:preparation of black phosphorus nanosheets（BPNSs）from black phosphorus crystal（BPC）by liquid exfoliation method.Then use NH2-PEG-FA to modify it;After loading with the anti-tumor drug DOX,the nanoparticles were loaded into GEL/PCL electrospinning by injection pump.Finally,a BP@DOX-PEG-FA-GP electrospinning scaffold with photothermal response is formed.The main advantage of this design is that the black phosphorus nanosheets are protected by PEG and electrospinning to prevent their rapid degradation.After exposure to near-infrared light,black phosphorus will degrades and produce photothermal effect.Then the doxorubicin released after the degradation can work together to treat cancer.At the same time,the gelatin produced by electrospinning degradation and the phosphate produced by black phosphorus degradation can synergistically promote wound healing in the treatment site.The morphology,structure and preparation process of nanocomposite particles and electrospinning scaffolds were characterized by transmission electron microscope,scanning electron microscope,fourier transform infrared spectrum and Zeta potential analyzer.The drug release behavior and the photothermal effect of electrospun scaffolds was studied in vitro,it is shown that the electrospun scaffolds with near infrared laser response has been successfully constructed.Next,the antitumor and wound healing efficiency of BP@DOX-PEG-FA-GP electrospinning scaffolds was studied in vitro.The anti-tumor efficacy of the electrospun scaffold was verified by cytotoxicity assay（cck-8）granule endocytosis assay（confocal flow cytometry microscopy）and apoptosis assay（cell morphology observation）.The cell compatibility test（cck-8）and apoptosis test（cell morphology observation）verified the good function of the scaffold in promoting wound healing.Then,the anti-tumor efficacy of BP@DOX-PEG-FA-GP electrospinning scaffolds was studied in vivo.Anticancer efficiency of the electrospinning scaffolds in vivo was evaluated by One-step TUNEL staining,hematoxylin and eosin staining（H&E）about morphological changes and apoptosis of tumor tissue.The electrospinning scaffolds was proved to be effective in promoting wound healing in vivo by using qrt-pcr,western blot,immunofluorescence staining and masson trichromatic staining.The results showed that the electrospinning scaffold loaded with black phosphorus nanoparticles with near-infrared light response was successfully constructed.It was proved that the black phosphorus electrospinning scaffold can induce apoptosis of melanoma cell by in vitro and in vivo experiments.But for normal skin cells,it has good biocompatibility can promote the proliferation and adhesion.Thus,the electrospinning scaffold can effectively target treat for melanoma and promote wound healing.This provides new ideas for the treatment and prognosis of melanoma. |
