Lilarutide Affects The Occurrence And Development Of Diabetic Periodontitis Through The JAK2/STAT3 Signaling Pathway

In recent years,the number of patients with periodontitis with diabetes has been increasing,and some studies have shown that there is a close correlation between diabetes and periodontitis.Therefore,finding a drug that can both treat diabetes and improve periodontitis has become a research hotspot.Liraglutide is a clinical drug for the treatment of type 2 diabetes due to its protective function of pancreatic islet cells.The drug has also been shown to have anti-inflammatory and osteogenic effects.Objective:the purpose of this study was to investigate the therapeutic effect of Liraglutide on diabetic periodontitis in vitro and to explore its mechanism.Methods:human periodontal membrane cells were identified by inverted microscope,immunofluorescence and alizarin red staining.The CCK-8 assay determined the optimal drug concentration and time to establish an in vitro model of diabetic periodontitis.The effect of Liraglutide on IL-6 and TNF-αmRNA and protein in diabetic periodontitis model was detected by qRT-PCR and ELISA.qRT-PCR,ELISA and Western blot were used to investigate the mechanism of Liraglutide on diabetic periodontitis.Results:the cells were long fusiform and radially arranged.CCK-8 results showed that high concentrations of AGEs and Pg-LPS inhibited the proliferation of human periodontal membrane cells,while low concentrations of AGEs and Pg-LPS promoted the proliferation of human periodontal membrane cells.100μg/mL AGEs,1μg/mL Pg-LPS treatment for 24 h had no significant effect on the proliferation activity of human periodontal membrane cells.Therefore,100μg/mL AGEs,1μg/mL Pg-LPS treatment for 24 h was the optimal drug concentration and time to establish an in vitro model of diabetic periodontitis.The results of qRT-PCR and ELISA showed that the expression of inflammatory factors IL-6 and TNF-αin the diabetic periodontitis model was up-regulated,and the expression of IL-6 and TNF-αcould be down-regulated by the treatment with Liraglutide.The results of qRT-PCR,ELISA and Western blot showed that the expression of JAK2 and STAT3 were up-regulated in the diabetic periodontitis model,which could be reversed after treatment with Liraglutide.The expression of JAK2,STAT3,IL-6 and TNF-αwas upregulated when coumermycin A₁ C-A₁（JAK2 agonist）was added.Conclusion:Liraglutide has therapeutic effect on diabetic periodontitis by inhibiting JAK2/STAT3 signaling pathway.Liraglutide is expected to be an emerging treatment for diabetic periodontitis...