| Objective : This study was conducted to investigate the relationship between complement and LN pathological types and SLEDAI scores by measuring serum complement levels in LN patients,and to provide new ideas for the diagnosis and treatment of LN.Methods::Serum from 50 patients hospitalized for renal puncture biopsy for LN from November2021 to October 2022 in the Department of Nephrology of the First People’s Hospital of Yunnan Province and 30 healthy controls from the physical examination centre were collected to detect serum H factor,mannose-binding lectin（MBL）,membrane attack complex(Serum levels of H-factor,Mannose-binding Lectin（MBL）,Membrane Attack Complex（MAC）and complement C1 q were measured and general information,disease duration,laboratory data and SLEDAI scores were collected.（Due to the small number of type Ⅱ and Ⅲ cases,this study was combined into one pathological type and was recorded as type Ⅱ and Ⅲ.）Results:1.Healthy controls,male:female=15:15,mean age（43.2±13.42）years（24-67 years）;LN patients,male:female=13:37,mean age（34.7±14.65）years（14-71 years）;LN patients were classified according to SLEDAI score: 27 cases in the severe activity group（≥15 points）,13 cases in the moderate activity group（10-14 points）,5 cases in the mild activity group（5-9 points）,and 5 cases in the stable activity group（<5 points）.The patients with LN were classified according to the SLEDAI score as follows: 27 in the severe activity group（≥15 points）,13 in the moderate activity group（10-14 points）,5 in the mild activity group（5-9 points）and 5 in the stable activity group（<5 points）;the patients with LN were classified according to the renal pathological typing: 8 in types Ⅱ and Ⅲ;14 in type Ⅳ;4 in type V;7 in type V+Ⅲ;17 in type V+Ⅳ.2.Serum H factor（1177.213 ± 328.154）ng/ml and C1q（16.40 ± 5.45）mg/dl were significantly lower in the LN group than in the healthy control group,and serum MAC（7.76 ± 2.02）pg/ml was significantly higher than in the healthy control group（all P values <.05）;serum MBL（21.07 ± 6.13）ng/ml was statistically different from that of the healthy control group.healthy controls was not statistically different（P>0.05）.3.serum H-factor and C1 q were significantly lower in type Ⅱ,Ⅲ and Ⅳ LN patients than in healthy controls,and serum MAC was significantly higher than in healthy controls（all P values less than 0.05）.Type V serum H factor was significantly lower than that of healthy controls,serum MAC was significantly higher than that of healthy controls（all P values less than 0.05）,and serum C1 q was not statistically different from that of healthy controls（P>0.05）.Serum H-factor,C1 q and MAC were not statistically different from healthy controls in type V+Ⅲ（all P values > 0.05）.Type V+Ⅳ serum C1 q was significantly lower than that of healthy controls,serum MAC was significantly higher than that of healthy controls（all P values less than 0.05）,and serum H factor was not statistically different from that of healthy controls（P>0.05）.4.There was a significant negative correlation between SLEDAI score and serum C1 q level（r=-0.301,P<0.05）,and no correlation with serum MBL,MAC and H-factor level（P>0.05）.Conclusions: 1.complement classical and alternative pathways have important roles in promoting LN pathogenesis.2.in type Ⅱ,Ⅲ and Ⅳ LN pathogenesis,both complement classical and alternative pathways are involved;in type V LN pathogenesis,mainly complement alternative pathway is involved;in V+Ⅳ LN pathogenesis,mainly complement classical pathway is involved.3.LN disease activity is associated with activation of complement classical pathway.4: lectin Role of the pathway in LN pathogenesis is unclear;insufficient evidence for complement involvement in V+Ⅲ LN pathogenesis... |
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