| Escherichia coli diarrhea in piglets,as the main disease leading to the death of weaned piglets,brings huge losses to the swine industry.At present,in the clinical treatment of piglets Escherichia coli diarrhea,antibiotics are often used for treatment,but due to the use of antibiotics,there will be great drug resistance and drug residues.In addition,Escherichia coli has many different serotypes and fimbriae genes,so the application of vaccines difficult to achieve a comprehensive prevention.There is a precedent for the use of traditional Chinese medicine to treat diseases in my country,and there is no problem of drug residue and drug resistance in traditional Chinese medicine.Therefore,E.coli diarrhea in piglets can be treated with traditional Chinese medicine.The purpose of this study is to screen out traditional Chinese medicine prescriptions for the treatment of the disease.The experiment contents and results are as follows:First,ten suspected Escherichia coli strains were isolated from the feces collected from some swine farms in Jilin Province,and identified by the methods of medium selection,microscopic examination,biochemical experiment and 16S r RNA PCR amplification.The identification results were all Escherichia coli.Then the virulence gene ST was determined by PCR amplification and the animal pathogenicity experiment was used to screen out enterotoxigenic Escherichia coli,numbered E1.The enterotoxin Escherichia coli was selected to establish a mouse diarrhea model,and fifty experimental mice were randomly divided into five groups,and intraperitoneal injection of Escherichia coli was 5×10~8cfu/m L inoculation group,4×10~8cfu/m L inoculation group,3×10~8cfu/m L inoculation group,2×10~8cfu/m L inoculation group and control group.Scoring by observing diarrhea,feed intake and mental state,the results showed that the best dose of Escherichia coli causing diarrhea in mice was 4×10~8cfu/m L.Fourteen Chinese medicines were selected by consulting related literature,and the minimum inhibitory concentration(MIC)of each Chinese medicine on E1 was determined using the test tube double dilution method.According to the determined MIC value and the contraindications of traditional Chinese medicine,fourteen kinds of traditional Chinese medicines were combined into three traditional Chinese medicine prescriptions and named as prescriptions one,two and three,with enrofloxacin as the positive control group,and three groups of traditional Chinese medicines as the experimental group for in vitro antibacterial experiments.The results show that the antibacterial effect of Chinese medicine prescription one is better,both of which are greater than prescription two and prescription three.Three traditional Chinese medicine prescriptions and western medicine enrofloxacin were administered to mice with diarrhea according to groups,and the therapeutic effect of Chinese medicine on mice with E.coli diarrhea was determined.The experimental design is as follows:60 Kunming mice were random Ly divided into 6 groups:Chinese medicine prescription one group,Chinese medicine prescription two group,Chinese medicine prescription three group,enrofloxacin group,diarrhea group,blank group,and each mouse’s abdominal cavity before administration Inject 0.5m L of 4×10~8cfu/m L Escherichia coli bacteria liquid.Except for the blank group,all mice in the other groups had diarrhea,and they were given 0.2 m L of the corresponding drug every day for 7 days.During the treatment,the food intake,body weight,cure rate,apparent rate,fecal scoring,and mortality of each group of mice were recorded every day.Before treatment and 3 days,5 days,and 7 days during treatment,blood was collected from diarrhea mice,serum was prepared,and immune indexes were determined.At each time point,1mice of each group were sacrificed and the intestinal tube was taken to make pathological sections to observe the small intestine.The pathological changes.The results showed that the average weight gain of mice in a group of traditional Chinese medicine prescriptions was significantly different from that of the second and third groups of traditional Chinese medicine prescriptions(P<0.05).Compared with the model group,there were significant differences between one group,two groups,and three groups of traditional Chinese medicine prescriptions(P<0.05),and the difference between one group,two groups and three groups of traditional Chinese medicine prescriptions was significant compared with the blank group(P<0.05).The average weight gain was lower than that of the western medicine group;the average feed intake of mice in the group of Chinese medicine prescriptions was significantly different from that of the second and third groups of Chinese medicine prescriptions(P<0.05).Compared with the western medicine group,the average feed intake of mice in a group of traditional Chinese medicine prescriptions was not significantly different(P>0.05),which was greater than that of the model group and was significantly different(P<0.05),and was less than that of the blank group and was significantly different(P<0.05).Compared with the diarrhea group,the fecal scoring of the four drug-taking groups were significantly different(P<0.05).In the traditional Chinese medicine treatment group,there was no significant difference between the traditional Chinese medicine prescriptions one,two and three(P>0.05).The fecal scoring of the traditional Chinese medicine group There was no significant difference between the Western medicine group and the western medicine group(P>0.05).the cure rate and effective rate of the medication group were higher than that of the diarrhea group,and the mortality rate was lower than that of the model group.The cure rate of the traditional Chinese medicine prescription group one was higher than that of the prescription group two and the prescription group three.The level of IL-2,IL-6,IL-8 and TNF-αin the four groups of medication gradually decreased during the treatment process.After the treatment,the four groups of medication were significantly different from the diarrhea group(P<0.05).The levels of IL-2,IL-6,IL-8 and TNF-αin the prescription group one were significantly different from those in the prescription group two and three(P<0.05),but there was no significant difference compared with the western medicine group(P>0.05).The pathological section showed that the small intestinal villi of the mice in the group of Chinese medicine prescriptions were intact,and the mucosal epithelium was not damaged,which ensured the normal digestive function of the body,which was similar to the treatment results of the western medicine group;Part of the small intestinal villi of mice in groups two and three of Chinese medicine prescriptions fell and broke,and inflammatory cells appeared;The small intestinal villi of the mice in the diarrhea group were severely broken and almost all fell off.The results of the above data show that:Chinese medicine prescription one,Chinese medicine prescription two,and Chinese medicine prescription three can all have a better therapeutic effect on E.coli diarrhea to a certain extent,but prescription one has the best effect.In summary,this experiment isolated E.coli from the feces collected from some pig farms in Jilin Province,screened out pathogenic E.coli,and successfully established a mouse diarrhea model.According to the minimum inhibitory concentration and compatibility of traditional Chinese medicines,fourteen traditional Chinese medicines are composed of three compound prescriptions;through the measurement of various indicators,the results show that the therapeutic effect of traditional Chinese medicine prescription one on mice E.coli diarrhea is better than traditional Chinese medicine prescription two,Chinese medicine prescription three,indicating that Chinese medicine prescription one is more effective in treating Escherichia coli diarrhea. |
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