Study On Transcriptome Difference Of Renal Cortical Cells Of Bactrian Camel Induced By High Salt And High Glucose

In order to adapt to the harsh environment,bactrian camel has the ability of high salt adaptation and blood glucose regulation,which is different from common mammals.However,the metabolic mechanism of tolerance to high salt and high glucose in Bactrian camel remains unclear.In this study,Bactrian camel was selected as the research object,and the transcriptome sequencing(RNA-Seq)of Bactrian camel renal cortical cells was carried out by high-throughput sequencing technology.The genes and metabolic pathways regulated by high salt and high glucose were screened,so as to reveal the adaptation mechanism of Bactrian camel to high salt and high glucose formed in the long-term natural evolution process.The renal cortex of Bactrian camel was collected and renal cortical cells were cultured.The experiment was divided into two groups: hypertonic treatment and high glucose treatment.In the first group,renal cortical cells were treated with hypertonic stress(HS)and control,and transcriptome sequencing was performed.In the second group,renal cortical cells of Bactrian camel were treated with high glucose(HG)and low glucose(LG),and transcriptome sequencing was performed.The results of sequencing were used to screen the differential expression genes(DEGs),and go enrichment and KEGG enrichment analysis were used to annotate the gene function and gene pathway of DEGs.The results of hypertonic treatment showed that there were 4854 differentially expressed genes(DEGs)in Bactrian camel renal cortical cells induced by high salt,of which 3407 were up-regulated and 1447 were down-regulated.Go enrichment analysis showed that DEGs were significantly enriched in G-protein coupled receptor activity,receptor binding,nucleic acid binding transcription factor activity,plasma membrane,ion channel activity,immune response and other pathways.KEGG pathway enrichment indicated that DEGs were significantly enriched in Signal transduction,Metabolism of cofactors and vitamins,Signaling molecules and interaction,Transport and catabolism,Immune systemand other pathways.Four differential genes were selected for Quantitative Real-time PCR,and the expression trend of the four differential genes was consistent with the overall trend of RNA-Seq,which proved that the high-throughput sequencing results were accurate and reliable.The results of high glucose treatment showed that a total of 458 DEGs were found in the renal cortex cells of Bactrian camel induced by high glucose.among which the up-regulated genes included Camk2 B,CRYAA and VNN1.High glucose stimulation can activate PI3K-Akt signaling pathway,AGE-RAGE signaling pathway,TGF-βsignaling pathway and NF-κB signaling pathway,which are related to diabetic nephropathy.RNA-Seq was used to understand the gene expression and tissue metabolic pathway characteristics of Bactrian camel renal cortical cells in high salt and high glucose environment,so as to reveal the physiological mechanism of Bactrian camel resistance to high glucose and high salt,and lay a foundation for further study of molecular biology of camel response to high salt stress and high glucose stress.