Oxidative Stress Of Camel Kidney Cells Cultured With High-glucose And High-temperature

Kidney is an important metabolic organ in the body,it can not only remove the metabolites in the body,but also have endocrine function and secrete hormones to participate in the regulation of physiological function.According to the transcriptomic data obtained from the two treatments of water-deprivation stress and salt-stress,the kidney has a trend of upregulated expression of antioxidant genes and the regulatory role of non-coding RNA involvement in antioxidant regulation.This experiment validates the antioxidant effects of the antioxidant genes at the cellular level by the transcriptome data.First,the renal cortex and renal medulla cells of the Bactrian camel were cultured in vitro,followed by high-glucose and high-temperature stress simulation culture of the cells,and finally the expression of antioxidant genes under the adversity culture was detected.The results as follows:1.Under high-glucose stress,ROS content in renal cortex cells was significantly increased at 5.5,10,15 and 25 mmol/L glucose concentration,and ROS content in renal medulla cells was significantly increased at 15,25 and 40 mmol/L glucose concentration.The m RNA expression levels of CAT,SOD2,SOD3,Gpx1,Gpx3,SLC6A1 and SLC14A1 in renal cortical cells were obviously significant difference at 10 mmol/L glucose concentration.The m RNA expression levels of Gpx1,Gpx3,Gpx4,GR,PEX5 L,PCBP2and ACLY were extreme significant difference at 40 mmol/L glucose concentration.The m RNA expression levels of CAT,Gpx1 and Gpx3 in renal medulla cells were obviously significant difference at 10mmol/L glucose concentration.The m RNA expression levels of CAT,SOD1,Gpx1 and Gpx3 were obviously significant difference at 40 mmol/L glucose concentration.SLC6A1 m RNA expression was extreme significant difference at25 mmol/L glucose concentration.2.Under high-temperature stress,ROS content in renal cortex cells increased significantly at 40℃,42℃ and 45℃,and in renal medulla cells at 42℃ and 45℃.In renal cortical cells,the m RNA expression of SOD2 was obviously significant difference at 40 ℃,42 ℃ and 45 ℃,SOD3 at 42 °C and 45 °C.Gpx1 was significant difference at45℃.The m RNA expression of Gpx3 at 45 °C was extreme significant difference,Gpx4 at 40 °C and 42 °C.The m RNA expression of CAT was obviously significant difference3.42 ℃ and 45 ℃.In renal medulla cells,the expression of SOD1 was significant difference at 40 °C,42 °C and 45 °C.SOD2 and Gpx1 were extreme significant difference difference at 45 ℃.According to the ROS detection results in cells and the differential expression of antioxidation-related genes,it is known that renal cortical cells are sensitive to glucose concentration.When deviating from the blood glucose concentration,ROS content will increase,and antioxidant genes and antioxidation-related genes will be up-regulated to produce OS.The renal medulla cells are not sensitive to glucose concentration,ROS will increase only when the glucose concentration is extremely deviated from the blood sugar concentration,and the corresponding antioxidation-related genes will also be up-regulated to produce OS.Both renal cortical cells and renal medulla cells are sensitive to high-temperature stress.ROS content in cells increases when the temperature is higher than that in vivo,and antioxidant genes are also up-regulated to produce OS.