Study On The Species Of Mongolian Medicinal Plants For Antimicrobial Use In Inner Mongolia And The Feeding Effect Of Inula Helenium

After banning the use of feeding antibiotics completely,the problem of replacing feeding antibiotics should be solved urgently.In accordance with the purpose of inheriting ethnic medicine and making full use of regional resources,in the context of replacing feeding antibiotics,this study took Mongolian medicine plants with antibacterial effect in Inner Mongolia as the research object,and made statistics on their distribution,richness,utilization parts and methods to screen out the plants with priority for use of Mongolian medicine.The effects of 10 Mongolian medicinal plants on the growth of Escherichia coli and Lactobacillus plantarum were studied by in vitro microbial experiments.Combined with modern breeding to explore the feasibility of mixed feeding application of Mongolian medicine plants and silage,for the development of breeding industry and livestock and poultry animal health,livestock product safety and ecological environmental protection,economic stability and development services.This study mainly includes the following three parts:1.Screening Mongolian medicine plants with "gummicidal",antimicrobial and disease-resistant microorganisms in Inner Mongolia by means of literature review,number of plant specimens inquiry,expert consultation and other methods.The results showed that a total of 99 species,including Compositae,Ranunculaceae,etc.The used parts are mainly the aboveground parts;The method of internal use,direct modification mainly.16 species of Mongolian medicine plants can be fed and utilized in priority.2.10 kinds of Mongolian medicine plants were selected and extracted by thermal extraction method.The extracts were added into the culture medium of Escherichia coli and Lactobacillus plantarum at 5 concentrations(0%,1%,2%,3%,4%),and the number of colonies was observed and recorded.The test results show that: In vitro culture test of Escherichia coli,compared with the control group,the extracts of Thymus serpyllum 1%,Taraxacum mongolicum 1%,Sophora flavescens1%,Atractylodes Lancea 2%,Artemisia annua 3%,Inula helenium 1%,Melilotus suaveolens 3% and Platycladus orientalis 1% had the best inhibitory effect on Escherichia coli,while the other Mongolian medicine plants had no significant effect(P>0.05).In vitro culture experiment of Lactobacillus plantarum,compared with the control group,the growth of Lactobacillus plantarum was significantly promoted when the extract was added at the I.helenium concentration of 1%(P<0.05),while the growth of Lactobacillus plantarum was not significantly affected or even inhibited by the others.3.Selection of I.helenium made into powder,through 60 mesh screen.A total of15 female Tan sheep were randomly divided into 3 groups with 5 sheep in each group,which were control group(without the addition of I.helenium),0.5% I.helenium group(diet 0.5% I.helenium /kg)and 1% I.helenium group(diet 1% I.helenium /kg).The pre-feeding period was 7 days and the normal feeding period was 14 days.The effects of pre-feeding period on the health status,serum biochemical and immune of Tan sheep were studied.The results showed that the supplementation of 1% I.helenium could improve the feed intake,protect the liver function,and promote the increase of serum of total protein(TP),albumin(ALB),immunoglobulin A(Ig A),immunoglobulin G(Ig G)and immunoglobulin M(Ig M)of Tan sheep.In conclusion,there are abundant species of Mongolian medicinal plants for antimicrobial use in Inner Mongolia,but the resources of wild medicinal plants are short at present.Therefore,it is feasible to give priority to the cultivated species for feed additives.This study provides a basis for the selection of species of Mongolian medicinal plants for antimicrobial use.In combination with the in vitro bacteriostatic test,the Mongolian medicine plants screened preliminarily were selected as feed additives.The results of feeding test on Tan sheep showed good performance,and it has a prospect of replacing the development and utilization of feeding antibiotics.This study provides a new way for the application of Mongolian medicine plants in the field of forage in Inner Mongolia.