| Yinchenhao decoction is recorded in Treatise on Febrile Diseases written by Zhang Zhongjing,which is composed with artemisia,gardenia and rhubarb.Some studies have shown that Yinchenhao decoction possesses the efficacy on anti-liver injury,prevention of fatty liver and anti-liver fibrosis,and is commonly used for the treatment of jaundice,acute viral hepatitis,intrahepatic cholestasis and other evidence of dampness and heat in clinic,which has a broad application prospect for treating liver diseases.The toxicity of gardenia can be reduced by adding licorice on the basis of Yinchenhao decoction.The prepared oral liquid can be used in the treatment of duck viral hepatitis.In this experiment,we firstly screened the raw materials of ten origins of modified Yinchenhao oral liquid,and then selected the best origins for the optimization of the preparation process,finally studied the quality control standards of the oral liquid.The results were as follows:(1)Screened of raw materials from 10 regions According to the main producing area,growing environment and market sales,the four raw materials of Artemisia Scoparia Herba,Gardeniae Fructus,Rheia Radix Et Rhizoma and Glycyrrhizae Radix Et Rhizoma were selected from 10 producing areas respectively.The results showed that the highest content of chlorogenic acid was found in Artemisia Scoparia Herba from Shanxi Yuncheng,the highest content of geniposide in Gardeniae Fructus from Hubei,the highest content of total anthraquinone in rhubarb from Shaanxi,and the highest content of glycyrrhizin in licorice from Gansu Gulang.So the herbs from these four origins were selected for subsequent preparation process and quality control studies.(2)Study on preparation process of modified Yinchenhao oral liquid In order to determine the scientific and reasonable preparation process conditions,this experiment carried out three extraction processes: water extraction,alcohol extraction,water extraction and alcohol precipitation.The extraction rate and the content of geniposide were used as indicators.Combined with the actual production,cost and other factors to determine the best process.The results showed that the best preparation process of modified Yinchenhao oral liquid was water extraction,which was,adding 12 times of water after 2 times of water absorption,soaking for0.5 hour,decocting for 1 hour each time,and decocting for 2 times.(3)Qualitative examination of modified Yinchenhao oral liquid The characters,relative density and p H of modified Yinchenhao oral liquid were examined by traditional detection method.The main components of oral liquid which are geniposide,emodin,and mono-ammonium glycyrrhizinate were identified qualitatively by thin-layer chromatography.The results showed that the modified Yinchenhao oral liquid was dark brown,moderate viscosity,slightly sweet flavor.The relative density was 1.06-1.10 mg/m L(20 ℃),and the p H was 4.87-4.90.In the chromatogram of the test product,the same brown spots were observed in the positions corresponding to the chromatograms of the geniposide reference substance and the gardenia reference medicinal material;the same yellow fluorescent spots were detected in the positions corresponding to the chromatograms of the emodin reference substance and the rhubarb reference medicinal material;the position corresponding to the chromatograms of the mono-ammonium glycyrrhizinate reference substance and the licorice reference medicinal material were shown the same dark brown spots.(4)Qualitative determination of modified Yinchenhao oral liquid The main components of geniposide,emodin and mono-ammonium glycyrrhizinate in modified Yinchenhao oral liquid were tested by high performance liquid chromatography and the content limit was determined.The results showed that the mobile phase of geniposide was acetonitrile: 0.1% phosphoric acid water(15:85),and the detection wavelength was 238 nm.The mobile phase of emodin was methanol: 0.1% phosphoric acid water(85:15),and the detection wavelength was 254 nm.The mobile phase of mono-ammonium glycyrrhizinate was acetonitrile: 0.1% phosphoric acid water(37:63),and the detection wavelength was 250 nm.The chromatographic column was Hadesil C18-K(4.6×250 mm,5 μm),the flow rate was 1m L/min,the column temperature was 30 ℃,and the sample was injected 10 μL.The contents of geniposide,emodin and glycyrrhizin in the oral liquid should not be less than 1.09 mg,7.12 μg and 0.73 mg per 1 m L,respectively. |
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