| The flowering and fruiting period of winter strawberries are mostly in winter.In addition,the use of facilities in greenhouses makes the winter cultivation of strawberries in facilities with insufficient sunlight more vulnerable to low temperature and weak light damage.Great economic and social significance.In this test,the most widely cultivated winter strawberry variety ’Hongyan’(Fragaria × ananassa cv.Benihoppe)potted seedlings in Sichuan was used as a test material and planted in a plastic greenhouse.When the greenhouse environment continued to have a temperature of ≤10 ℃ during the day and ≤5 ℃ at night Spray strawberry plants with different concentrations of 24-Epibrassinolide(EBR),Diethyl aminoethyl hexanoate(DA-6),24-Epibrassinolide+Diethyl aminoethyl hexanoate(EBR+DA-6).Determination of photosynthetic characteristics,chlorophyll,SOD,POD,CAT activity,MDA content,plant height,shortened stem thickness,dry and fresh weight of plants,and single fruit weight,aspect diameter,color,soluble sugar,titration Acid,ascorbic acid,soluble protein,total phenol,total flavonoids,anthocyanin content,and clear concentrations of EBR,DA-6,EBR+DA-6 on photosynthesis,antioxidant enzyme activity,plant growth potential,and fruit quality of strawberry leaves The effects of low-temperature and low-light stress on the optimal concentration of hormones on winter strawberry growth were further screened.The results showed that:1.Winter strawberry production areas in the Sichuan Basin are affected by extreme winter weather.Winter plastic greenhouses have low temperature,short light time,and large radiation loss.The limiting factors of photosynthetic rate are mainly non-stomatal factors.Low temperature and low light will reduce strawberry photosynthetic rate and affect its Yield and quality.2.Under low temperature and weak light stress,the application of appropriate amount of EBR,DA-6,EBR+DA-6 can effectively improve the net photosynthetic efficiency,transpiration and stomatal conductance of the leaves,reduce the concentration of intercellular carbon dioxide,and increase the SOD enzyme and POD The activity of enzymes and CAT enzymes and the content of soluble protein enhance the growth potential of plants,which is beneficial to the coloring of strawberry fruits and helps to improve the quality of strawberry fruits.It is comprehensively found that the single application of EBR and DA-6 is less effective than the combined application of EBR+DA-6 on various indicators of strawberry.3.Under the conditions of low temperature and low light,A1B1(0.5μmol / L EBR+0.139 mmol / L DA-6)combined with EBR+DA-6 had the best effect on the photosynthesis,cell membrane permeability and growth potential of strawberry plants,all of which were significant Higher than the control(P <0.05),A2B2(1 μmol / L EBR+0.186 mmol / L DA-6)had the best accumulation of strawberry fruit color,soluble solids,soluble sugar,and ascorbic acid,and the titratable acid content decreased.The antioxidant substances anthocyanin,total phenol and total flavonoids of the fruit were significantly higher than the control(P <0.05). |