| Korla Fragrant Pear is a characteristic agricultural product in Xinjiang,which is mainly planted in southern Xinjiang.It is an important part of local agricultural economy and Xinjiang characteristic forest and fruit industry.Korla Fragrant Pear is a cross pollinated fruit tree,pollination and fertilization directly affect the yield and quality of the fruit.At present,the main pollination methods used in production are:natural pollination,artificial dipping pollination,artificial shaking pollination,etc.These pollination methods have some problems,such as easily affected by weather conditions,unclear safety of pollination pollen,neven vitality of pollen,high pollination cost,low pollination efficiency,etc.In order to solve the pollination problems of Korla Fragrant Pear in flowering stage,this paper studied the preservation technology of pollinated pollen at room temperature,the detection method of Fire blight of pear in pollinated pollen and the pollen liquid parameters suitable for UAV liquid pollination1.The effect of 0.5%Chitosan on pollen germination in vitro of Yali pear,the results show that:0.5%chitosan aqua concentration of 0.5ml/L-1ml/L does not affect the in vitro germination rate of Yali pear pollen,but inhibited the growth of pollen tubes.When the concentration was higher than 1 ml/L,with the increase of chitosan concentration,the pollen germination rate and pollen tube growth of Yali pear were inhibited,and the inhibition degree was proportional to the concentration.When the concentration reached 2.8 ml/L,the pollen did not germinate.The results showed that 0.5%chitosan could inhibit the pollen germination of Yali Pear in vitro.2.With 0.5%chitosan aqueous solution concentration of 2.8ml/L,3ml/L,3.5ml/L,4ml/L,respectively,the Yali pollen was stored at 4℃,15℃,25℃for 1d,3d,5 days,the pollen vigor was tested by the in vitro germination method,the results showed that the germination rate of pollen stored in 0.5%chitosan aqueous solution at 4°C for 1 day was not significantly different from that of the control,but the length of the pollen tube was significantly reduced.With the increase of the storage temperature and the extension of the storage time,the pollen germination rate and the length of the pollen tube were significantly reduced.It shows that0.5%chitosan aqueous solution basically has no preservation effect on the pollen of Yali pear.3.Using primer E/primer F on the chromosome of P Erwinia amylovora and three pairs of primers p29A/p29B、AJ75/AJ76、PEANT1/PEANT2 on the plasmid of p EA29,the conventional PCR,duplex PCR and nested PCR systems for the detection of Erwinia amylovora in Yali pear pollen were established and the sensitivity of the established PCR system was used to detect the simulated samples of Yali pear pollen.The results showed that the sensitivity of the combination of primers Primer E/Primer F and PEANT1/PEANT2 for double PCR was 5.4×103 CFU/Reaction,and the sensitivity of the combination of primers Primer E/Primer F and p29A/p29B and 4 pairs primers of conventional PCR was 5.4×102CFU/Reaction,nest PCR first round primer p29A/p29B second round primer AJ75/AJ76 combination sensitivity is 5.4×101 CFU/Reaction,nest PCR first round primer p29A/p29B second round primer PEANT1/PEANT2 combination and Nest PCR first round primer AJ75/AJ76 the second round primer PEANT1/PEANT2 combination sensitivity is 5 CFU/Reaction.4.The results of optimization of parameter combination of Korla fragrant pear UAV liquid pollen solution and economic benefit analysis show that:the optimal combination of pollen solution parameters is3L/667m~2 of pollen solution,pollen dilution ratio 1:500,and application of pollen solution immediately.The combination Korla fragrant pear inflorescence fruit setting rate is 88.57%,flower fruit setting rate is 30.79%,and the pollination cost is 82 yuan/667m~2,which saves 158 yuan/667m~2 and 308 yuan/667m~2 respectively compared with artificial dipping powder and artificial powder shaking. |
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